the pseudomonas aeruginosa transcriptome in planktonic cultures and static biofilms using rna sequencing生物膜中的绿脓杆菌转录组浮游文化和静态使用rna序列.pdfVIP

The Pseudomonas aeruginosa Transcriptome in Planktonic Cultures and Static Biofilms Using RNA Sequencing ¨ 1 1 1 1 3 Andreas Dotsch *, Denitsa Eckweiler , Monika Schniederjans , Ariane Zimmermann , Vanessa Jensen , 2 2 ¨ 1,3 Maren Scharfe , Robert Geffers , Susanne Haussler 1 Chronic Pseudomonas Infections, Helmholtz Center for Infection Research, Braunschweig, Germany, 2 Genome Analytics, Helmholtz Center for Infection Research, Braunschweig, Germany, 3 Pathophysiology of Bacterial Biofilms, Twincore, Center for Clinical and Experimental Infection Research, joint venture of the Helmholtz Center for Infection Research and the Medical School Hannover, Hannover, Germany Abstract In this study, we evaluated how gene expression differs in mature Pseudomonas aeruginosa biofilms as opposed to planktonic cells by the use of RNA sequencing technology that gives rise to both quantitative and qualitative information on the transcriptome. Although a large proportion of genes were consistently regulated in both the stationary phase and biofilm cultures as opposed to the late exponential growth phase cultures, the global biofilm gene expression pattern was clearly distinct indicating that biofilms are not just surface attached cells in stationary phase. A large amount of the genes found to be biofilm specific were involved in adaptation to microaerophilic growth conditions, repression of type three secretion and production of extracellular matrix components. Additionally, we found many small RNAs to be differentially regulated most of them similarly in stationary phase cultures and biofilms. A qualitati