the role of interleukin-1 in the pathogenesis of human intervertebral disc degenerationinterleukin-1的作用在人类椎间盘退变的发病机制.pdfVIP

Available online /content/7/4/R732 Vol 7 No 4 Open Access Research article The role of interleukin-1 in the pathogenesis of human Intervertebral disc degeneration Christine Lyn Le Maitre, Anthony J Freemont and Judith Alison Hoyland Division of Laboratory and Regenerative Medicine, School of Medicine, University of Manchester, Manchester, UK Corresponding author: Judith Alison Hoyland, judith.hoyland@manchester.ac.uk Received: 29 Oct 2004 Revisions requested: 3 Dec 2004 Revisions received: 16 Feb 2005 Published: 1 Apr 2005 Arthritis Research Therapy 2005, 7:R732-R745 (DOI 10.1186/ar1732) This article is online at: /content/7/4/R732 © 2005 Le Maitre et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/ 2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract In this study, we investigated the hypotheses that in human immunopositivity for these proteins, with the exception of IL- intervertebral disc (IVD) degeneration there is local production 1Ra, increased with severity of degeneration. We have also of the cytokine IL-1, and that this locally produced cytokine can shown that IL-1 treatment of human IVD cells resulted in induce the cellular and matrix changes of IVD degeneration. increased gene expression for the matrix-degrading enzymes Immunohistochemistry was used to localize five members of the (MMP 3 (matrix metalloproteinase 3), MMP 13 (matrix IL-1 family (IL-1α, IL-1