the role of the yap5 transcription factor in remodeling gene expression in response to fe bioavailabilityyap5转录因子的作用在重构基因表达以应对菲生物利用度.pdfVIP

The Role of the Yap5 Transcription Factor in Remodeling Gene Expression in Response to Fe Bioavailability 1 1 1 1 2 Catarina Pimentel , Cristina Vicente , Regina Andrade Menezes , Soraia Caetano , Laura Carreto , Claudina Rodrigues-Pousada1* ´ ´ 1 Instituto de Tecnologia Quımica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal, 2 Department of Biology, Center for Environmental and Marine Studies, Universidade de Aveiro, Aveiro, Portugal Abstract The budding yeast Saccharomyces cerevisiae has developed several mechanisms to avoid either the drastic consequences of iron deprivation or the toxic effects of iron excess. In this work, we analysed the global gene expression changes occurring in yeast cells undergoing iron overload. Several genes directly or indirectly involved in iron homeostasis showed altered expression and the relevance of these changes are discussed. Microarray analyses were also performed to identify new targets of the iron responsive factor Yap5. Besides the iron vacuolar transporter CCC1, Yap5 also controls the expression of glutaredoxin GRX4, previously known to be involved in the regulation of Aft1 nuclear localization. Consistently, we show that in the absence of Yap5 Aft1 nuclear exclusion is slightly impaired. These studies provide further evidence that cells control iron homeostasis by using multiple pathways. Citation: Pimentel C, Vicente C, Menezes RA, Caetano S, Carreto L, et al. (2012) The Role of the Yap5 Transcription Factor in Remodeling Gene Expression in Response to Fe Bioavailability. PLoS ONE 7(5): e37434. doi:10.1371/journal.pone.0037434 Editor: Yoshiaki Tsuji, North Carolina State University, United States of America Received December