the single-nucleotide resolution transcriptome of pseudomonas aeruginosa grown in body temperature铜绿假单胞菌生长的单核苷酸决议转录组体温.pdfVIP

The Single-Nucleotide Resolution Transcriptome of Pseudomonas aeruginosa Grown in Body Temperature 1. 2,3. 2. 4,5 1 Omri Wurtzel , Deborah R. Yoder-Himes , Kook Han , Ajai A. Dandekar , Sarit Edelheit , E. Peter Greenberg4, Rotem Sorek 1"*, Stephen Lory 2"* 1 Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel, 2 Department of Microbiology and Immunobiology, Harvard Medical School, Boston Massachusetts, United States of America, 3 Division of Respiratory Diseases, Boston Children’s Hospital, Boston, Massachusetts, United States of America, 4 Department of Microbiology, University of Washington School of Medicine, Seattle, Washington, United States of America, 5 Division of Pulmonary and Critical Care Medicine, University of Washington School of Medicine, Seattle, Washington, United States of America Abstract One of the hallmarks of opportunistic pathogens is their ability to adjust and respond to a wide range of environmental and host-associated conditions. The human pathogen Pseudomonas aeruginosa has an ability to thrive in a variety of hosts and cause a range of acute and chronic infections in individuals with impaired host defenses or cystic fibrosis. Here we report an in-depth transcriptional profiling of this organism when grown at host-related temperatures. Using RNA-seq of samples from P. aeruginosa grown at 28uC and 37uC we detected genes preferentially expressed at the body temperature of mammalian hosts, suggesting that they play a role during infection. These temperature-induced genes included the type III secretion system (T3SS) genes and effectors, as well as the genes responsible for phenazines biosynthesis. Using genome- wide transcription