the stereochemistry of complex polyketide biosynthesis by modular polyketide synthases系列复杂酮化合物的立体化学生物合成酮化合物合成酶.pdfVIP

Molecules 2011, 16, 6092-6115; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Review The Stereochemistry of Complex Polyketide Biosynthesis by Modular Polyketide Synthases David H. Kwan 1,* and Frank Schulz 2,3,* 1 Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver B.C., V6T 1Z1, Canada 2 Fakultät für Chemie, Chemische Biologie, Technische Universität Dortmund, Otto-Hahn-Straße 6, 44221 Dortmund, Germany 3 Max-Planck-Institut für Molekulare Physiologie, Abteilung für Chemische Biologie, Otto-Hahn- Straße 11, 44227 Dortmund, Germany * Authors to whom correspondence should be addressed; E-Mails: dkwan@chem.ubc.ca (D.H.K.); frank3.schulz@tu-dortmund.de (F.S.); Tel.: +1-604-822-9300 (D.H.K.); +49-231-133-2429 (F.S.); Fax: +1-604-822-9126 (D.H.K.); +49-231-133-2498 (F.S.). Received: 8 June 2011; in revised form: 6 July 2011 / Accepted: 18 July 2011 / Published: 20 July 2011 Abstract: Polyketides are a diverse class of medically important natural products whose biosynthesis is catalysed by polyketide synthases (PKSs), in a fashion highly analogous to fatty acid biosynthesis. In modular PKSs, the polyketide chain is assembled by the successive condensation of activated carboxylic acid-derived units, where chain extension occurs with the intermediates remaining covalently bound to the enzyme, with the growing polyketide tethered to an acyl carrier domain (ACP). Carboxylated acyl-CoA precursors serve as activated donors that are selec