the struggle to detect circulating dna难以检测循环dna.pdfVIP

Available online /content/10/3/142 Commentary The struggle to detect circulating DNA Sacha Zeerleder Sanquin Research at CLB, Department of Immunopathology, Plesmanlaan 125, 1066 CX Amsterdam, The Netherlands Corresponding author: Sacha Zeerleder, s.zeerleder@sanquin.nl Published: 16 May 2006 Critical Care 2006, 10:142 (doi:10.1186/cc4932) This article is online at /content/10/3/142 © 2006 BioMed Central Ltd See related research by Rhodes et al., http:/content/10/2/R60 Abstract octamer of two copies each of histones H2A, H2B, H3 and In various diseases, such as cancer, autoimmune disease, sepsis H4, around which a stretch of helical DNA 146 base pairs in or myocardial infarction, elevated levels of circulating DNA can be length is wrapped. Oligonucleosomes are composed of measured. However, its predictive value is under debate. variable amounts of mononucleosomes connected by intact Circulating DNA in plasma is protein-bound (nucleosomal) DNA. linker DNA with a variable length of 15 to 100 base pairs Quantification of circulating DNA can be performed by real-time containing a ‘linker’ histone H1. Once released into the quantitative PCR or immunological methods such as ELISA. The circulation, nucleosomes seem to be protected by their diagnostic value of both methods can be impaired by inappropriate handling of the samples. Assessment of circulating DNA in structure from further degradation by endonucleases [7]. patients admitted to the intensive care unit offers a tool for predicting mo