甲壳聚多糖降脂降胆固醇实验研究（Chitosan, polysaccharide, lipid-lowering, cholesterol lowering, experimental study）.doc

甲壳聚多糖降脂降胆固醇实验研究（Chitosan, polysaccharide, lipid-lowering, cholesterol lowering, experimental study） Chitosan, polysaccharide, lipid-lowering, cholesterol lowering, experimental study Update: 2010-06-08: click on the storage masuhira Yin Shumei In high fat high cholesterol animal model induced by two methods, chitasan at 2.14 g/kg and 2.86 g/kg showed some fat, cholesterol lowering effect, at the same time, also increased high density lipoprotein cholesterol and low density lipoprotein cholesterol. Keywords chitosan polysaccharide; cholesterol lowering; cholesterol lowering; 1 experimental materials 1.1 drugs: chitosan polysaccharide (batch number 960801), the elimination of bile amines (as a positive control, purchased from Jiangsu Province Peoples Hospital); before the experiment, the two drugs were CMC (1%) into the required concentration of dissolved liquid. 1.2 animals: Kunming mice and SD rats (provided by the Nanjing medicine, Su Zhi 93011). 2 experimental methods and results 2.1 effects of yolk milk on high cholesterol in mice 60 male mice, weighing 20 + 1 g, were randomly divided into 6 groups, 10 rats in each group. The chitasan 3 groups, respectively by 1.61 g/kg, 2.14 g/kg, 2.86 g/kg orally; cholestyramine group (positive control) at 2.5 g/kg orally; blank control group and model group were fed with equal volume of 1% CMC, 1 /d, 5 consecutive D, to 1 last irrigation after 2 h respectively, by intraperitoneal injection of 75% egg yolk milk 0.3 ml/ only (control group except). After 20 h, the blood serum triglyceride, Cholesterol Reagent box, kit, low density lipoprotein (box were produced by Cholesterol Reagent Dongou biological engineering company reagent instrument factory), serum cholesterol (TC) were measured in each animal, triglyceride (TG) and low density lipoprotein cholesterol (HDL-C, LDL-C) content. The results are shown in table 1. Table 1 Effects of Chitosan on hyperlipidemia induced by egg yolk milk (SD, mg/dl) Group dose (g/kg) TC, TG, HDL-C,