组织学与胚胎学课本复习重点 免费1（Histology and Embryology textbook review key free 1）.docVIP

组织学与胚胎学课本复习重点 免费1（Histology and Embryology textbook review key free 1） 1. Introduction to histology The procedure of paraffin sectioning: sampling, fixation, dehydration, embedding and slicing. Hematoxylin eosin (H-E) staining: hematoxylin and eosin are alkaline reagents, which can change the eosinophilic substances into blue purple. Eosin is an acidic reagent, which can make the alkaline substances change to pale red. Silver affinity: when silver is dyed with nitric acid, the tissue can be reduced to black by the reduction of silver ion directly; Silver: when silver is dyed with nitric acid, the tissue can not be reduced directly to black by silver ions, and it needs to be added as a reducing agent to develop color. Ultrathin section: 50-80nm. grasps the concept of high electron density and low electron density (Textbook P4). PAS reaction: sugar + periodate oxidant + aldehyde + indicator (polysaccharide and glycoprotein were positive for PAS reaction). 2 characteristics of the epithelial tissue, cells densely arranged, interstitial cells; polarity: free surface and basal surface; no blood vessels, sensory nerve endings rich; the distribution of cavity surface coating on the surface, or lined in cavity organs; function: protection, secretion, absorption and excretion. 3. Classification of epithelial tissue: divided into two groups: covering epithelium and glandular epithelium by function. In addition, there are a few specialized epithelia in the body, such as sensory epithelium, myoepithelial, and reproductive epithelium. 4. The covering epithelium is classified and named according to the number of layers of its constituent cells and the morphology of the cells (which are superficial layers of the stratified epithelium). (Textbook P13 Table2-1) added: the outer surface of the gastric wall (single layer squamous epithelium) 5, grasp the special structure of epithelial cells: Free surface: Compare, microvillus, with, cilium. (P17) Flank: Explain, all, cell, junctions,