武大2009年考研_分子生物学真题及答案（Wuhan University graduate in 2009 _ molecular biology Zhenti and answers）.docVIP

武大2009年考研_分子生物学真题及答案（Wuhan University graduate in 2009 _ molecular biology Zhenti and answers） Xing Wenxuan included finishing 2009 graduate school of molecular biology First, the noun explanation: 1, Regulatory gene Regulatory gene: a gene that encodes a regulatory protein that can bind to a sequence of controls. Regulatory proteins are divided into repressor proteins and activator proteins that bind to the operon to regulate gene transcription. 2, Real-time PCR Real time quantitative PCR: refers to the PCR index during amplification by continuous monitoring the change of fluorescence signal intensity to instant determination of specific product quantity, and infer the initial amount of target gene, does not need to separate the PCR products. The concentration of reactants can be measured at intervals equal to the time, and relatively accurate data can be obtained. 3, Nick translation Notch translation method: when an incision is made on one strand of a double stranded DNA molecule, E.coli DNA polymerase I can connect nucleotides to the 3hydroxyl end of the notch. At the same time, the enzyme is from 5to 3 exonuclease activity, can be removed from the 5terminal nucleotide incision. Due to the cut nucleotide and at the same time the 3end up incision incision so that the nucleotide, moving along the DNA chain, which used instead of non radioactive radioactive nucleotide nucleotide, the radioactive isotope incorporation into new chain synthesis. 4 Generic promoter common promoter of a gene in many segments may be repeated with other genes, such as the common gene promoter region, can make different gene expression by the same promoter. 5 Histone code histone code The hypothesis of histone code suggests the existence of second types of human genetic code, This code, called the histone code, is the master switch that controls the genes turn on.. Histone codes help control the expression of human genes. The histones in the code are proteins that make up chromatin. Chroma