深圳赤潮中霍乱弧菌噬菌体的分离筛选及生物学特性分析.PDF

微生物学通报 JAN 20, 2010, 37(1): 1218 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 深圳赤潮中霍乱弧菌噬菌体的分离筛选及 生物学特性分析 * 蔡俊鹏 孙丽滢 (华南理工大学轻工与食品学院 广东 广州 510640) 摘 要: 本文以从鲍鱼肠道中分离出的霍乱弧菌 SWBC-a 为宿主菌, 采用双层平板法从2004 年夏季 深圳大梅沙海域赤潮海水样品中分离得到 10 株噬菌体。通过对分离出的噬菌体效价、RTD 值的测 定、形态特征的观察及对属内外细菌的交叉裂解谱的研究, 筛选出 3 株裂解谱较宽的强效噬菌体。 选取其中 2 株高效裂解噬菌体 PsaA 和 PsaH 做生物学特性分析, 证明除镁离子有利于噬菌体裂解外, 温度、pH 值、紫外照射、柠檬酸钠对噬菌体裂解都有不同程度的抑制。本研究结果为进一步利用 噬菌体研发用于消除霍乱弧菌的微生态制剂和诊断食源性霍乱弧菌提供了理论基础。 关键词: 噬菌体, 霍乱弧菌, 生物学特性, 理化因素 Isolation and Characterization of Bacteriophages of Vibrio cholera from the Red Tide Waters in Shenzhen * CAI Jun-Peng SUN Li-Ying (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, Guangdong 510640, China) Abstract: Using Vibrio cholerae seperated from the intestines of abalones as host, ten bacteriophages of Vibrio cholera were isolated by double-layer plate method from sea water sample from the Red tide at Dameisha of Shenzhen in 2004. Three broad lysis spectrum strains were found by detecting microbiological assay, RTD, the character of plaque morphology under electron microscope and the lysis spectrum. PsaA and PsaH were selected among all the strains as the representation to study the biological characteristics. The results proved that heat inactivation, pH, ultraviolet rays and sodium citra