AIHA患者外周血Th1／Th2、Tc1／Tc2亚群探究及意义.docVIP

AIHA患者外周血Th1／Th2、Tc1／Tc2亚群探究及意义 　 作者：吴伟全，孙炎华，邱少玲 【关键词】 自身免疫性溶血性贫血 　　［摘要］ 目的：研究自身免疫性溶血性贫血(autoimmune haemolytic anaemia，AIHA)患者外周血Th1／Th2、Tc1／Tc2亚群极化状态，分析AIHA的免疫学发病机制，为AIHA的临床治疗提供新的理论依据。方法：收集AIHA患者及健康对照组外周抗凝静脉血，分离纯化T细胞。以Cy5标记的抗CD4、CD8单抗和PE标记的CD30单抗作双色流式细胞术，分析AIHA患者Th／Tc、Th1／Th2、Tc1／Tc2亚群的比例变化。结果：与正常对照组相比，AIHA患者外周血CD4+细胞百分率显著降低(Plt;0．05)，CD8+细胞百分率无明显变化(Pgt;0．05)，CD4+/CD8+比例明显降低(Plt;0．05)，CD4+CD30-T、CD4+CD30+T细胞百分率明显下降，CD4+CD30-T／CD4+CD30+T和CD8+CD30-T／CD8+CD30+T比例均明显升高(Plt;0．05)，而CD8+CD30-T及CD8+CD30+T细胞百分率无明显变化(Pgt;0．05)。结论：AIHA患者外周血存在细胞免疫功能失调，T细胞亚群极化状态发生改变，呈明显Th1／Tc1型细胞优势；Th1／Tc1型细胞介导的细胞免疫可能与AIHA的免疫学发病机制有关。 　　 　　［关键词］ 自身免疫性溶血性贫血；T细胞亚群；研究 　　Study on Th1／Th2、Tc1／Tc2 Subsets on Peripheral Blood of the Patients with Autoimmune Haemolylic Anaemia and It’s Significance 　　Abstract:Objective To investigate the change of Th1like and Th2like cells balance in autoimmune haemolytic anaemia(AIHA)patients．Methods The ratios of Th／Tc，Th1／Th2 and Tc1／Tc2 inperipheral blood T cells were analyzed by immunofiuorescence staining and bicolor flow cytometry(FCM) in vitro．Results Compared with the ratios of Th1／Th2 (48．76％±6．17％)and Tc1／Tc2(18．90％±4．12％)in healthy people，the ratios of Th1/Th2 (56．21％±5．95％)and Tc1／Tc2(23．09％±3．31％) in AIHA patients increased obviously．FCM analysis showed that average percentages of Th，Th1，Th2，Tc，Tc1 and Tc2 are (22．31％±6．51％)，(21．92％±6．42％)，(0．37％±0．14％)，(32．12％±6．15％),(30．95％±5．45％) and (1．34％±0．84％) in AIHA patients versus (38．24％±5．82％)，(38．01％±5．47％)，(0．90％±0．16％)，(31．25％±5．63％)，(29．72％±5．20％) and (1．52％±0．68％) in healthy people．The average percentages of Th,Th1 and Th2 decreased obviously．While the average percentages of Tc,Tc1 and Tc2 did not changed．Conclusion The ratios of Th1／Th2 and Tc1／Tc2 in peripheral blood T cells were increased obviously in AIHA patients．It suggested that cellular immunity in AIHA patients is shifted to Th1 typeimmunity，which may relate to the mechanism of AIHA． 　　Key words：Autoimmune haemolytic anaemia； T cell subsets; Study