乐胃饮对脾虚型IBS兔胃肠激素及氧自由基影响.docVIP

乐胃饮对脾虚型IBS兔胃肠激素及氧自由基影响 　 作者：徐珊 晋丽君 谭涛 张永生 潘永明 陈方明 ; 【摘要】; 　　［目的］观察中药复方乐胃饮对脾虚型IBS兔胃肠激素及氧自由基的影响，探讨乐胃饮治疗脾虚型IBS的可能作用机制。［方法］两月龄白毛黑眼兔（WHBE兔）36只，雌雄各半，随机分为6组：正常组、模型组、香砂六君丸组、丽珠肠乐组、乐胃饮低剂量组、乐胃饮高剂量组。除正常组外，其余WHBE兔采用湿热环境应激联合番泻叶灌胃加饥饱失常复合方法致脾虚泄泻型IBS模型。从模型复制的中期开始各用药组预防给药，至造模结束。观察各组一般情况，测定血清NO、NOS、MDA、SOD含量及结肠黏膜组织中SP含量。［结果］模型组WHBE兔血清NO、NOS、MDA含量、结肠黏膜SP阳性表达较正常组均显著升高（Plt;0.01或Plt;0.05），血清SOD活力明显降低（Plt;0.01）。药物干预后，乐胃饮高剂量组血清NO含量较模型组明显降低（Plt;0.01）；除乐胃饮低剂量组外，余用药组血清NOS活力、血清MDA含量与模型组比较均明显降低（Plt;0.01或Plt;0.05）、血清SOD活力明显增加（Plt;0.01或Plt;0.05）；各用药组结肠黏膜SP阳性表达较模型组均明显降低（Plt;0.01或Plt;0.05）。［结论］乐胃饮能有效调节脾虚型IBS兔胃肠激素的异常分泌及氧自由基的异常表达，这是乐胃饮治疗脾虚型IBS的可能作用机制。 【关键词】; 肠易激综合征 胃肠激素 氧自由基 乐胃饮 　　Abstract：［Objective］ To observe the effect of Leweiyin of traditional Chinese compounds prescription on gastrointestinal hormones and oxygen free radicals in IBS rabbits of spleen deficiency， and to explore the possible mechanism for the treatment of IBS with spleen deficiency.［Methods］ 36 twomonthold rabbits with white feather and black eyes （WHBE rabbit），half male and half female，were randomly divided into 6 groups：normal group，model group，Xiangsha Liujun pill group， Lizhu Changle group， lowdose Leweiyintreated group，highdose Leweiyintreated group.Except the normal group， others were made into IBS model of spleen deficiency which was induced by damp heat stress and gavage with Fanxieye as well as method of either hunger or gorge.The drug groups were administered preventively from the mediumterm of the model reproduction to the end of modeling.The rabbits of each group were observed.NO，NOS，MDA，SOD in serum and SP in colonic membrane were detected.［Results］ NO，NOS，MDA in serum and SP in colonic membrane significantly more increased than the normal group（Plt;0.01or Plt;0.05），and the activity of SOD in serum decreased significantly （Plt;0.01）.After drug intervention， compared with the model，the serum NO content of highdose Leweiyintreated group decreased greatly（Plt;0.01）.In addition to lowdose Leweiyintreated g