乳腺癌组织中核转录因子NFκB及抑癌基因p53表达及其意义.docVIP

乳腺癌组织中核转录因子NFκB及抑癌基因p53表达及其意义 　 作者：邓元, 张学斌, 王鸿雁, 张冠军, 张群岭, 王敏 【摘要】 目的 探讨乳腺癌组织中核转录因子NFκB和抑癌基因p53的表达及意义。方法 用免疫组织化学方法检测NFκB p65和p53蛋白在58例乳腺癌和20例乳腺增生症组织中的表达。结果 乳腺癌中NFκB表达明显高于乳腺增生症组织，NFκB表达强度与组织学分级和腋窝淋巴结转移有关，与患者年龄、肿瘤大小、组织学分型无关。乳腺癌中p53蛋白表达明显高于乳腺增生症，p53蛋白的表达与乳腺癌组织学分级有关，与患者年龄、肿瘤大小、组织学分型、淋巴结转移无关。NFκB p65与p53蛋白在乳腺癌组织中表达呈正相关（r=0.302，P＜0.05）。结论 提示乳腺癌中NFκB和p53蛋白的表达与乳腺癌的发生发展有关。 【关键词】 乳腺癌；NFκB；p53蛋白；免疫组织化学 　　　　Abstract：Objective To study the expression and significance of nuclear transcription factorκB(NFκB) and tumor suppressor gene p53 protein in breast cancer.Methods Immunohistochemical staining was used to detect the expression of NFκB p65 and p53 protein in 58 cases of breast cancer and 20 cases of proliferative breast disease.Results The expressive degree of NFκB in breast cancer was significantly higher than in proliferative breast disease. The positive intensities of NFκB was associated with histologic grading and axillary lymph node metastasis，but was not associated with age, tumor size, and histological type. The expressive degree of p53 is significantly higher than in proliferative breast disease. The positive intensities of p53 were associated with histologic grading, but were not associated with age, tumor size, histological type and axillary lymph node metastasis. The expression of NFκB and p53 protein were significantly positive correlated in breast carcinoma （r=0.302，P＜0.05）.Conclusion It is suggested that the abnormal expression of NFκB and p53 are associated with the pathogenesis and development of breast cancer. 　　Key words：Breast carcinoma; NFκB; Tumor suppressor gene p53; Immunohistochemistry 　　 　　0 引言 　　核转录因子NFκB是一种具有多向调节功能的转录因子，可调控细胞增殖、分化、凋亡及恶性转化[1,2]，NFκB p65是NFκB的活化成分，在肿瘤的发生、发展中起重要作用。p53基因是一种重要的抑癌基因，它的突变、缺失或失活可导致肿瘤的发生。我们采用免疫组化法检测乳腺癌组织中NFκB和p53蛋白的表达，探讨二者间的相互关系及预后意义。 　　1 材料与方法 　　1.1 材料 　　选取本院1994～1999年间58例乳腺癌切除标本存档蜡块，患者均为女性，年龄30～86岁，平均51.8岁。肿瘤直径1～10cm；腋窝淋巴结转移阳性38例，阴性20例。术前均未做过治疗。按WHO标准对各病例进行分型和分级，其中浸润型导管癌47例，浸润型小叶癌5例，髓样癌4例，粘液腺癌2例。组织