去除白细胞在海南地区临床输血中应用现状.docVIP

去除白细胞在海南地区临床输血中应用现状 　【摘要】 目的 探讨应用白细胞过滤器对全血或SAGM（氯化钠-腺嘌呤-葡萄糖-甘露醇）悬浮红细胞进行白细胞去除，比较白细胞去除前后血液的质量及临床应用现状，预防非溶血性输血反应。方法 将全血或悬浮红细胞使用白细胞过滤器去除白细胞，采用血细胞计数仪来分析白细胞去除和红细胞回收情况，并且比较去除白细胞组与未去除白细胞组发生的临床非溶血性输血反应率。结果 在400ml全血或悬浮红细胞中白细胞去除率gt;99％（99.26％～99.41％），红细胞回收率gt;90％（90.97％～92.32％），血红蛋白回收率gt;99％（99.62％～99.84％）’’血小板去除率gt;38％（38.11％～39.89％）’’非溶血性输血反应发生率为0.35％，较未去白细胞组发生的非溶血性输血反应发生率4.1％明显降低，两者比较差异有显著性(Plt;0.01)。结论 经过去除白细胞的血液成分制品能显著降低非溶血性反应率、白细胞携带病毒相关疾病的传播、由HLA抗体引起同种免疫反应，真正满足了临床输血的需要。 【关键词】 去除白细胞；输血；海南地区；非溶血性输血反应 The current application of depleting leukocyte in the clinical transfusion in Hainan province 【Abstract】 Objective To investigate the application of leukocytes removal filters for depleting leukocytes in whole blood or SAGM red cell suspensions，the change of blood quality before and after filtered by leukocytes removal filters comparative with blood quality application in the clinical，preventing febrile non-hemolytic transfusion reactions(FNHTR).Methods The leukocytes were removed by leukocytes removal filters from whole blood or SAGM red cell suspensions.The extent of leukocyte depletion and red cell recovery was based on cell counts.Trace leukocytes and red cell were counted by using a blood cytometer’’Comparative with non-hemolytic blood transfusion reactions before and after filtered by leukocytes removal filters.Results The leukocytes were removed more than 99％(99.26％～99.41％) in whole blood or in SAGM red cell suspensions prepared from 400ml whole blood.Red cell recovery exceeded 90％(90.97％～92.32％).The hemoglobin recovery exceeded 99％(99.62％～99.84％)’’and the platelets were removed more than 38％(38.11％～39.89％).The reaction rate of non-hemolytic blood transfusion was 0.35％ after using leukocytes removal filters and it was 4.1％before .There was significant difference (Plt;0.01).Conclusion Leukoreduction of cellular blood products by filtration has been shown to decrease the incidence of febrile non-hemolytic transfusion reactions ‘‘transmission of leukocyte-associated