地灵丹汤对单侧输尿管梗阻大鼠模型肾间质纤维化防治作用.docVIP

地灵丹汤对单侧输尿管梗阻大鼠模型肾间质纤维化防治作用 　 作者：宦红娣, 张景红, 郑士荣, 孟巍巍, 张军力 【摘要】 　　目的：研究中药复方地灵丹汤对单侧输尿管梗阻（unilateral ureteral obstruction，UUO）致肾间质纤维化模型大鼠肾间质纤维化的防治作用及其可能机制。 　　方法：将60只SD大鼠随机分为假手术组、模型组、依那普利组及地灵丹组。采用UUO法建立肾间质纤维化大鼠模型，观察血尿素氮、血清肌酐和24 h尿蛋白定量及肾组织病理改变，并采用免疫组织化学法检测转化生长因子β1（transforming growth factorβ1, TGFβ1）、α平滑肌肌动蛋白（αsmooth muscle actin, αSMA）、纤维连接蛋白（fibronectin, FN）及层黏连蛋白（laminin, LN）的表达。 　　结果：第7天模型组肾间质大量炎症细胞浸润及胶原表达，TGFβ1、FN和LN表达面积百分率较假手术组明显增加（Plt;0.05）；地灵丹组间质纤维化面积较模型组减少（Plt;0.05）。第14天地灵丹组和依那普利组肾间质TGFβ1、αSMA、FN和LN表达面积百分率较模型组减少（Plt;0.05），BUN、SCr和24 h尿蛋白无明显差异。第21天地灵丹组SCr、肾间质纤维化和TGFβ1表达面积百分率较依那普利组和模型组下降（Plt;0.05）。 　　结论：地灵丹能减轻单侧输尿管梗阻模型大鼠的蛋白尿和肾间质纤维化，抑制TGFβ1、αSMA、FN和LN的表达。在较长时间应用后，地灵丹对模型大鼠的肾功能保护作用优于依那普利。 【关键词】 地灵丹; 肾间质纤维化; 转化生长因子β1; α平滑肌肌动蛋白; 纤维连接蛋白; 层黏连蛋白; 大鼠 　　Objective: To investigate the preventive and therapeutic effects and the possible mechanisms of Dilingdan Decoction (DLDD), a compound Chinese herbal medicine, on rats with renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction (UUO). 　　Methods: Sixty SD rats were randomly divided into shamoperated group, untreated group, enalapriltreated group and DLDDtreated group. Blood urea nitrogen (BUN), serum creatinine (SCr), urine protein quantization in 24 hours and pathological changes of the obstructed kidney were observed. The expressions of transforming growth factorβ1 (TGFβ1), alphasmooth muscle actin (αSMA), fibronectin (FN) and laminin (LN) were detected by immunohistochemical method and coloredmultimedia pathological image analysis system. 　　Results: Massive inflammatory infiltrates and collagen expression in renal interstitial in the untreated group were observed on the 7th day. Compared with the shamoperated group, percentages of area of TGFβ1, αSMA, FN and LN expressions in the untreated group were markedly increased (Plt;0.05), while the percentage of area of interstitial fibrosis was decreased in the DLDDtreated group as compared with the untreated group (Plt;0.05). On the 1