急性白血病患者SHP-1及c-kit基因表达及其临床意义.docVIP

急性白血病患者SHP-1及c-kit基因表达及其临床意义 　 作者：韩颖，罗建民，贾晓辉，王福旭，姚丽，杜行严 【摘要】 为了探讨造血细胞磷酸酶（SHP-1）基因和原癌基因c-kit在急性白血病（AL）患者中的表达及其与AL的发生及预后的关系，应用半定量逆转录-聚合酶链反应（RT-PCR）方法检测60例急性白血病患者及33名正常对照者单个核细胞的SHP-1、c-kit mRNA的表达。结果显示：SHP-1在AML细胞中表达阳性率由高至低依次为缓解组、初治组、复发组，c-kit表达阳性率则相反，各组与正常对照组相比有显著性差异（Plt;0.05），SHP-1在AML细胞中表达阳性率比ALL细胞高，有显著性差异（Plt;0.05），c-kit在AML细胞中表达阳性率比ALL细胞中高，有显著性差异（Plt;0.05）；SHP-1与c-kit表达成负相关（r＝-0.502，Plt;0.05）；30例初治AML患者SHP-1＋与SHP-1-的完全缓解率均有显著性差异（Plt;0.05），c-kit＋与c-kit-的完全缓解率均有显著性差异（Plt;0.05）。结论：SHP-1可能是一个潜在的抑癌基因，它负性调节c-kit基因而抑制肿瘤的发生，同时监测二者的表达可作为判断AL治疗转归的预测指标。 【关键词】 急性白血病； SHP-1； c-kit； 聚合酶链反应 　　Expression and Clinical Value of SHP-1 and c-kit in Acute Leukemia 　　AbstractThe aim of study is to investigate the expression of hematopoietic cell phosphatase (SHP-1) gene and c-kit pro-oncogene in acute leukemia(AL) and its impact on prognosis in AL. Semi-quantity reverse transcriptase-polymerase chain reaction(RT-PCR) was used to detect the expression of SHP-1 mRNA and c-kit mRNA in 60 AL patients and 33 normal controls(NC). The results showed that the positive rates of SHP-1 expression from high to low level were found orderly in complete remission group, newly diagnosed group and relapsed group, there was significance difference between each group and NC group（Plt;0.05）. The positive rates of c-kit expression were opposite order in each groups as compared with SHP-1. there was also significance difference between each group and NC group（Plt;0.05）. The positive rate of SHP-1 and c-kit expressions in AML was higher than that in ALL（Plt;0.05）, there was nagative correlation between expressions of SHP-1 and c-kit （r＝-0.502，Plt;0.05）; The difference between the complete remission rate in SHP-1 positive and in SHP-1 negative patients from 30 newly diagnosed AML patients was significant (Plt;005), the same result was found between c-kit+ complete remission and c-kit- complete remission. It is concluded that SHP-1 gene is a potentially anti-oncogene and inhibits the growing of tumor by nega