携载阿霉素磁性纳米Fe3O4颗粒制备及逆转多药耐药实验探究.docVIP

携载阿霉素磁性纳米Fe3O4颗粒制备及逆转多药耐药实验探究 　作者：孙茜，陈宝安，王雪梅，高峰，戴永援，程坚，丁家华，高冲，李景源，许文林，沈慧玲 【摘要】 为了建立一种磁性纳米Fe3O4颗粒携载聚合阿霉素的制备方法，探讨载药纳米颗粒对K562及其耐药株K562/A02的作用，用机械吸附聚合法在4℃、37℃下以不同药球比聚合24、48小时合成载药纳米颗粒，用噻唑蓝比色法检测细胞与载药纳米颗粒悬液孵育48小时后的生存率，并计算细胞抑制率。结果表明： 随着磁性纳米Fe3O4颗粒浓度增加，两种细胞抑制率均提高，聚合在4℃、48小时时的细胞抑制率分别高于37℃、24小时的细胞抑制率。 结论： 磁性纳米Fe3O4颗粒可通过机械吸附法携载阿霉素，聚合具有温度、时间依赖特性；载药纳米颗粒有逆转多药耐药作用。 【关键词】 磁性纳米颗粒 Preparation of Fe3O4magnetic Nanoparticles Loaded with Adriamycin and Its Reversal of Multidrug Resistance In Vitro Abstract To prepare Fe3O4magnetic nanoparticles loaded with adriamycin and investigate the reversal role of drugloaded nanoparticles in K562 and resistant cell line K562/A02, the drugloaded nanoparticles were prepared by using mechanical absorption polymerization at different conditions of 4℃ or 37℃ for 24 or 48 hours. The survival of cells cultured with drugloaded nanoparticles for 48 hours was detected by MTT assay, then the growth inhibition efficacy of cells was calculated. The results showed that the growth inhibition efficacy of both two cell lines was enhanced with increasing concentration of Fe3O4magnetic nanoparticles. The inhibitory ratio of two cell lines obtained at 4℃ and for 48 hours was significantly better than that at 37℃ and 24 hours. In conclusion, Fe3O4magnetic nanoparticles can load adriamycin by using mechanical absorption polymerization, but depended on proper temperature and time. Furthermore, drugloaded nanoparticles showed an ability reversing multidrug resistance. Key words magnetic nanoparticle; Fe3O4magnetic nanoparticle; Adriamycin; mechanical absorption polymerization; multidrug resistance 逆转多药耐药(multidrug resistance, MDR)、提高化疗敏感性对于恶性血液病的治疗具有积极意义［1］。纳米材料携载化疗药能够增强化疗敏感性、克服多药耐药。本研究在不同条件下将磁性纳米Fe3O4颗粒(Fe3O4magnetic nanopar ticles，Fe3O4MNPs)采用机械吸附法聚合阿霉素（adriamycin， ADM）［2，3］，考察适宜的聚合条件及载药纳米颗粒对K562及其耐药细胞株K562/A02的作用。 材料和方法 主要药剂和试剂 磁性纳米四氧化三铁(Fe3O4)颗粒由东南大学生物医学工程系生物电子学国家重点实验室提供，使用前将其用新鲜配制的RPMI 1640＋10％小牛血清培养液混合，超声混匀，制成分散均匀的Fe3O4MNPs胶体悬浮液；ADM为浙江海正药业公司产品，分散