柴苓归芪汤加蚓激酶对实验性肾病综合征大鼠肾功能保护作用.docVIP

柴苓归芪汤加蚓激酶对实验性肾病综合征大鼠肾功能保护作用 　 作者：张芬芳，王喜栋，范焕芳，陈志强，尹智炜，张伟 【关键词】 柴苓归芪汤 ［摘要］ 目的：探讨柴苓归芪汤加蚓激酶对实验性肾病综合征大鼠肾功能的保护作用。方法：采用尾静脉一次性注射阿霉素5 mg/kg制作肾病大鼠模型，随机分为模型组、辛伐他汀组、柴苓归芪组，并设正常对照组。造模1周后，予以药物干预，共用药5周。采用三氯乙酸法测定大鼠24 h尿蛋白含量；全自动生化分析仪检测血清总蛋白（total protein, TP）、白蛋白（albumin, Alb）、总胆固醇（total cholesterol, TC）、甘油三酯（triglycerides, TG）、高密度脂蛋白（highdensity lipoprotein, HDL）、低密度脂蛋白（lowdensity lipoprotein, LDL）、肌酐（creatinine, Cr）及血尿素氮（blood urea nitrogen, BUN）。光镜、电镜下观察肾组织病理形态学变化。结果：模型组与正常对照组比较，24 h尿蛋白定量及血清TC、TG、LDL、Cr、BUN均明显升高（P＜0.05或P＜0.01)；血清TP、Alb、HDL则明显降低（P＜0.01)。柴苓归芪汤组24 h尿蛋白定量及血清TC、TG、LDL、Cr、BUN均明显低于模型组（P＜0.05或P＜0.01)；而血清TP、Alb和HDL则明显高于模型组（P＜0.05或P＜0.01)。肾组织病理形态学观察：模型组光镜下部分肾小球呈局灶节段性硬化，近曲小管上皮细胞变性肿胀，部分小管腔内可见蛋白管型，间质可见散在的纤维增生；电镜下肾小球上皮细胞足突广泛融合。柴苓归芪汤组未出现肾小球硬化，肾小管间质病变明显减轻，肾小球上皮细胞足突少量融合。结论：柴苓归芪汤能够降低大鼠尿蛋白，调节脂质代谢紊乱，保护肾脏功能，延缓肾脏疾病进展。 　　［关键词］ 柴苓归芪汤; 阿霉素肾病; 蛋白尿; 脂质代谢; 高脂血症; 肾组织; 病理形态学 　　Protective effects of Chailing Guiqi Decoction combined with lumbrukinase on renal function in rats with adriamycin nephropathy 　　ABSTRACT Objective: To study the protective efffects of Chailing Guiqi Decoction (CLGQD) combined with lumbrukinase on renal function in rats with adriamycin nephropathy. Methods: Thirtysix SD rats were randomly divided into four groups: normal control group, untreated group, simvastatintreated group and CLGQD treated group. Adriamycin nephropathy was induced by intravenous injection with 5 mg/kg adriamycin. After sevenday treatment, quantitative measurement of 24h urine protein was determined with trichloroacetic acid, and serum total protein (TP), albumin (Alb), total cholesterol (TC), triglycerides (TG), highdensity lipoprotein (HDL), lowdensity lipoprotein (LDL), creatinine (Cr) and blood urea nitrogen (BUN) were assessed using automatic biochemistry analyzer. The pathomorphological changes of renal tissues were observed with light and electron microscopes. Results: In the untreated group, the 24h urine protein excretion, serum TC, TG, LDL, Cr and BUN were significantly higher than those