环氧化酶2抑制剂Nimesulide对膀胱癌T24细胞体外生长抑制作用.docVIP

环氧化酶2抑制剂Nimesulide对膀胱癌T24细胞体外生长抑制作用 　 作者：王勤章, 王蓉, 丁国富, 倪钊, 张国玺, 李应龙, 王新敏, 谢顺明 【摘要】 目的 观察环氧化酶2(COX2)抑制剂Nimesulide(NIM)对人膀胱癌T24细胞株体外生长的影响，并探讨其作用机制。方法 采用细胞形态学、MTT法、流式细胞术、吖啶橙溴化乙啶双染法（AOEB）等技术检测NIM对体外培养的人膀胱癌细胞株T24生长的抑制效应。结果 细胞形态学观察可见NIM对T24细胞生长具有明显的细胞毒作用；MTT法、流式细胞术分析显示NIM能明显抑制T24细胞的增殖，且呈剂量、时间依赖关系；细胞周期分析表明，NIM能使T24细胞G0/G1期细胞比例升高，S期和G2/M期细胞比例下降；流式细胞DNA直方图上出现典型的亚二倍体“凋亡峰”，细胞凋亡率与NIM作用时间和剂量相关；荧光显微镜检查显示NIM处理的T24细胞AOEB双染色后，细胞核内可见浓染致密的颗粒荧光，典型细胞可见新月形改变，固缩或片段化的核。结论 COX2抑制剂NIM对T24细胞生长具有明显的抑制作用，其作用机制可能与阻断细胞周期、诱导细胞凋亡等有关。 【关键词】 环氧化酶2；尼美舒利；T24细胞株；体外作用 　　The Inhibit Effect of the Cyclooxygenase2 Inhibitor Nimesulide on Human Bladder Cancer Cell Line T24 in Vitro 　　Abstract：Objective To investigate the growth effects of the selective cyclooxygenase2 inhibitor Nimesulide on human bladder cancer cell line T24 in vitro, and to clarify its probable mechanism.Methods The T24 cell line was culture in vitro, and exposed to a range of concentrations of Nimesulide at different time. The effect of COX2 inhibitor Nimesulide on the proliferation and apoptosis of human bladder cancer cell line T24 in vitro were observed by using cell morphology , MTT assay , flow cytometry, fluorescent microscopy.Results Significant cytotoxicity of cyclooxygenase2 inhibitor Nimesulide on human bladder cancer cell line T24 were observed by cell morphology. MTT assay and flow cytometry show that cyclooxygenase2 inhibitor Nimesulide significantly inhibited cell proliferation of T24 cell line in vitro in a dose and timedependent manner .Cellcycle analysis by flow cytometry suggested that Nimesulide induced a decrease in S and G2/M phase population and a significant increase in G0/G1 population compared with untreated T24 cells. And a typical subdiploid peak before Phase G0/G1 was detected by flow cytometry. The rates of apoptosis were correlated with the concentration of Nimesulide and time of action. A typical apoptosis morphology were also observed by fluorescent microscopy.Conclusion The selective cyclo