白藜芦醇对早期重症急性胰腺炎大鼠多器官组织氧自由基影响.docVIP

白藜芦醇对早期重症急性胰腺炎大鼠多器官组织氧自由基影响 　 作者：秦勇　马清涌　党晓燕　沙焕臣 【摘要】 　　目的 观察白藜芦醇对早期重症急性胰腺炎(SAP)大鼠器官组织SOD、MDA的影响，进一步研究白藜芦醇的作用机制。方法 将54只大鼠随机分为三组(假手术组、急性胰腺炎组、白藜芦醇治疗组各18只)。应用逆行胰胆管穿刺注射牛黄胆酸钠制备SAP大鼠模型，白藜芦醇溶液通过阴茎背静脉注射，术后4h剖杀,观察腹水量及胰、肝、肺、肾和肠壁组织的超氧化物歧化酶(SOD)、丙二醛(MDA)和组织学变化及白藜芦醇对其影响。结果 模型组的胰、肺、肝、肾、小肠组织SOD活性降低、而MDA水平增加；白藜芦醇可明显升高胰、肺、肝、肠、肾组织的SOD活性,降低各组织的MDA水平，同时白藜芦醇可减轻大鼠各器官组织病理损害，以胰腺组织为主。结论 氧自由基参与了SAP的病理生理过程。白藜芦醇可提高SAP大鼠多器官组织SOD活性、降低MDA水平而减轻组织脂质过氧化和组织器官功能损害。 【关键词】 重症急性胰腺炎；白藜芦醇；氧自由基；超氧化物歧化酶；丙二醛 　　Effect of resveratrol on oxygen free radicals of multiple organs　in rats with severe acute pancreatitis of early stage 　　ABSTRACT: Objective To observe the effect of resveratrol on superoxide dismutase and malondialdehyde of multiple organs in rats with severe acute pancreatitis of early stage. Methods A total of fiftyfour rats were divided into three groups (sham group, SAP model group and resveratroltreated group ). SAP model was made by injecting sodium taurocholate 0.1mL/100g to the pancreatic bile duct, and resvoratrol was given intravenously. The rats were sacrificed at four hours after inducing SAP model. The levels of MDA and SOD and histological changes of pancreas, lungs, liver, kidneys and small intestines were measured. Results The levels of MDA in pancreas, lungs, liver, kidneys and small intestine tissues in model group were significantly higher than those in sham group (Plt;0.05), while the level of SOD was significantly lower in the tissues involved. The levels of SOD in pancreatic and lung tissues in resveratroltreated group were significantly higher than those in model group, while the levels of MDA in pancreas, lungs, liver, kidneys and small intestines were significantly lower in the tissues concerned. Conclusion Oxygen free radicals participate in the process of pathological changes in SAP. Resveratrol might increase SOD activity and decrease MDA level, thus attenuating lipid peroxidation in multiple organs of SAP, especially in pancreas and lung tissues. 　　KEY WORDS: severe acut