神经节苷脂GM1及亚低温联合应用对新生鼠缺氧缺血性脑损伤保护.docVIP

神经节苷脂GM1及亚低温联合应用对新生鼠缺氧缺血性脑损伤保护 　 作者：王海英, 刘家浩, 柴斌英, 唐洪丽 【关键词】 神经节苷脂GM1；亚低温；脑缺氧；缺氧缺血性脑损伤；大鼠[中图分类号]R363,,,, 　　[摘要]目的 探讨神经节苷脂GM1与亚低温对新生大鼠缺氧缺血性脑损伤的保护作用。方法 用75只新生7d的SD大鼠制作缺氧缺血（HIBD）模型，随机分为假手术（A）组、模型（B）组、神经节苷脂GM1（C）组、亚低温（D）组、神经节苷脂与亚低温联合干预（E）组共5组，每组15只。观察不同干预方法对新生大鼠缺氧缺血后24h脑含水量、神经细胞死亡率及30d后学习和记忆能力的影响。结果 B组脑含水量、神经细胞死亡率显著高于A组，学习、记忆能力显著下降(Plt;0.05)，各干预组脑含水量、神经细胞死亡率下降，学习、记忆能力显著改善（Plt;0.05），而以E组改变更明显(Plt;0.05)。 结论 神经节苷脂GM1和(或)亚低温均可显著减轻HI后脑损伤，联合干预保护作用更佳。 　　[关键词]神经节苷脂GM1；亚低温；脑缺氧；缺氧缺血性脑损伤；大鼠[中图分类号]R363 　　Neuroprotective effects of ganglioside GM1 and mild hypothermia on hypoxicischemic brain damage in neonatal rats 　　Abstract: Objective To investigate the protective effects of ganglioside GM1 and/or mild hypothermia on hypoxicischemic brain damage in neonatal rats. Methods Seventyfive neonate rats(subjected to unilateral left carotid artery ligation and given 8%oxygen for 2h) were randomly allocated to five groups：sham operation group(A),model group(B),GM1 group(C),hypothermia group(D), GM1 and hypothermia group(E).Changes of brain edema and percentage of dead neurons(HDNP) at 24h and behaviors of learning and memory at 30d after HI insult were investigated. Results Brain edema and HDNP were significantly increased in group B compared with group A,whereas behaviors of learning and memory in group B were reduced significantly. Brain edema,HDNP and behaviors were improved in group C,D and E(Plt;0.05)，the most obvious changes was observed in group E(Plt;0.05). Conclusion Ganglioside GM1 and/or mild hypothermia have protective effects on HIBD,especially when ganglioside GM1 and mild hypothermia are used simultaneously. 　　Key words: ganglioside GM1; hypothermia; brain hypoxia; hypoxic ischemia encephalopathy; rats　(Modern Medical Journal,2007,35: 1720) 　　新生儿缺氧缺血性脑病（hypoxic ischemic encephalopathy,HIE）是指围生期窒息缺氧所导致的脑缺氧缺血性损害而出现的一系列临床脑病表现，是新生儿时期危害最大的常见病之一，常可引起不可逆的组织损伤，从而引起一系列神经系统后遗症。目前许多保护胎儿和新生儿免于缺氧缺血性脑损伤（HIBD）的药物或非药物的干预方法取得了长足的进步，但迄今尚无统一有效的治疗方案。近年来随着对HIE发病机制研究的不断深入，更多的研究焦点集中在寻找一些新的有效治疗措施