肾病综合征儿童淋巴细胞表型及细胞因子变化分析.docVIP

肾病综合征儿童淋巴细胞表型及细胞因子变化分析 　【关键词】 肾病综合征 　　摘要: 目的:通过对原发性肾病综合征(PNS)儿童外周血T淋巴细胞表型及细胞因子（白细胞介素-6、肿瘤坏死因子-α）监测，探讨不同患病时期PNS儿童T淋巴细胞表型及白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)变化的临床意义。方法：选取我院儿科门诊及住院诊断为PNS患儿共40例，年龄在2～15岁，分活动期组及缓解期组，活动期及缓解期各检测一次。对照组为经过体格检查为正常健康儿童，年龄在2～14岁，共20例。流式细胞仪检测淋巴细胞表型CD3、CD4、CD8的阳性细胞百分率；酶联免疫技术对细胞因子（IL-6、TNF-α）监测，组间进行比较t检验。结果：活动期与缓解期、对照组比较，CD4细胞数量明显降低(Plt;0.01)，CD8细胞数量明显升高(Plt;0.01)，CD4/CD8比值显著降低(Plt;0.01)，IL-6、TNF-α含量均显著升高(Plt;0.01)。缓解期IL-6与对照组比较显著性(Ｐlt;0.01)。结论：PNS患儿免疫功能明显下降,免疫的辅助功能降低，CD4、CD8、IL-6、TNF-α含量变化可作为判断PNS患儿病变活动或缓解和估计预后及治疗效果的一项参考指标。 　　关键词: T淋巴细胞表型；IL-6；TNF-α； PNS 　　The Variety Analysis of T-lymphocyte Type and Cell Factor of Nephropathy Syndrome Child 　　　Abstract:Objective：examineing the outer blood T-lymphocyte type and cell factor(interleukin 6,tumor necrosis factor) of the primordial nephropathy syndrome(PNS) infant,to study the clinic meaning when T-lymphocyte type and interleukin 6(IL-6),tumor necrosis factor(TNF-a) change of the different sicken period children.Method： 40 PNS children in the policlinic and be in hospital,age 2～15 years,were selected and divided into active period group and alleviative period group, examine T-lymphocyte type and cell factor once in different period.The normal group were composed of 20 healthy children checked in the policlinic,age 2～14 years. Use the cell hydro-apparatus examine the masculine percent of T-lymphocyte type CD3、CD4、CD8 cell, ELISA (enzyme linked immunosorbant assay)to examine cell factor,and compared different group with t-test. Result: Compare the normal group、 active period group and alleviative period group,CD4 cell amount obviously depress (Plt;0.01),CD8 cell amount obviously raise(Plt;0.01),CD4/CD8 ratio obviously depress (Plt;0.01), IL-6、TNF-αcontent obviously raise(Plt;0.01),. IL-6 be markedly raise compared the normal group to alleviative period group(Ｐlt;0.01). Conclusion: PNS child immunity function obviously depress, immunity assistant function also depress. The content change of CD4、CD8、IL-6、TNF-α