苯甲醇对红细胞冻干前海藻糖负载红细胞影响探究.docVIP

苯甲醇对红细胞冻干前海藻糖负载红细胞影响探究 　【摘要】 为研究苯甲醇对海藻糖负载红细胞的影响，在4℃条件下将红细胞孵育在浓度分别为10、30、50、100 mmol/L的苯甲醇海藻糖溶液中24小时，用氰化血红蛋白试剂盒测定海藻糖负载红细胞的溶血率，用硫酸蒽酮法检测红细胞内海藻糖浓度水平。结果表明：在100 mmol/L苯甲醇海藻糖溶液组，其红细胞内海藻糖浓度为72±12.98 mmol/L，与其它各组相比，有显著统计学差异（p=0.000）；溶血率为17.99±3.75%，与其它各组相比，有显著统计学差异（p=0.000）。结论：苯甲醇可提高海藻糖负载红细胞的负载率，随着苯甲醇浓度的升高红细胞海藻糖负载率也提高，100 mmol/L的苯甲醇浓度为可用浓度。 【关键词】 苯甲醇 Effect of Benzyl Alcohol on Trehaloseloading Red Blood Cells before Lyophilization Abstract This study was aimed to evaluate the effect of benzyl alcohol on trehaloseloading red blood cells (RBCs). The RBCs were incubated in 10, 30, 50 and 100 mmol/L concentrations of benzyl alcoholtrehaloe solution at 4℃ for 24 hours. The hemolysis rate of loaded RBCs was detected by using cyanohemoglobin kit, the intracellular trehalose level were assayed by sulfate anthrone method. The results showed that the intracellular trehalose concentration in group with 100 mmol/L benzyl alcohol was 72±12.98 mmol/L，compared with that in groups of 10, 30 and 50 mmol/L, the statistical difference were significant (p=0.000); the hemolysis rate of loaded RBCs in group with 100 mmol/L of benzyl alcohol was 17.99±3.75%, as compared with groups of 10, 30 and 50 mmol/L, the statistical difference was significant (p=0.000). It is concluded that benzyl alcohol can enhance the intracellular trehalose concentration. As concentration of benzyl alcohol ascends, the intracellular trehalose concentration increases.100 mmol/L benzyl alcohol may be proper for loading RBCs. Key words benzyl alcohol; red blood cell; trehalose J Exp Hematol 2007; 15(4):882-884 目前，输血已成为临床重要的治疗手段之一，据统计，90%的输血目的是为了补充红细胞。用现有的红细胞保存装备保存费用昂贵，不便运输，远远不能满足临床急救和突发事件或灾害事故的救援。冻干红细胞由于其性能稳定，可在常温下保存，保存时间长，保存费用低廉，易于再水化，输注方便，以固体干粉剂形式存在，体积小，便于运输，克服了目前血液保存的局限性，因此冻干红细胞的保存已成为研究的热点。 冻干红细胞的研究已有30多年的历史，进展缓慢.。2004年，Satpathy等［1］利用渗透非平衡原理和磷脂膜相变相结合原理将海藻糖导入到红细胞内。2005年，Satpathy等［2］将负载海藻糖的红细胞冰冻干燥，再水化后血红蛋白回收率达59%。这项研究，为冻干红细胞的临床应用迈出了重要一步。2005年，我们在Satpathy实验的基础上，改进某些实验方法，用海藻糖负载红细胞，并进行冻干保存研究，实验结果与Satpathy的实验结果接近