血浆VEGF CA153 CA125检测对乳腺癌诊断价值.docVIP

血浆VEGF CA153 CA125检测对乳腺癌诊断价值 　【摘要】 目的：探讨血管内皮细胞生长因子、CA153联合检测在乳腺癌诊断中的价值。方法：采取56例乳腺癌患者血浆标本作实验组，102例乳腺良性疾病血浆标本作对照组。VEGF采用生化比色法测定，CA153用化学发光免疫法检测。结果：VEGF、CA153对乳腺癌的敏感性分别为60.7%、58.9%，特异性分别为98.4%、96.3%，准确性分别为89.8%、88.1%。VEGF及CA153联合检测乳腺癌的敏感性为92.8%，特异性为88.8%，准确性为89.8%。联合检测与单项指标检测相比，敏感性明显提高。结论：血浆VEGF、CA153单项检测对乳腺癌的诊断均有较大意义，联合检测血浆VEGF及CA153能提高乳腺癌的诊断敏感性。 【关键词】 血管内皮细胞生长因子(VEGF)； CA153； 乳腺癌； 血 浆 　　Study on Value of Combined Analysis of VEGF CA153 CA125 in Diagning of Breast Cancer 　　　Abstract：Objective：To study the value of combined assay of VEGF，CA153 in the diagnosis of breast cancer．Method：56 patients with breast cancer were studied，and 102 cases with benign breast diseases were randomly selected as controls. Serum level of VEGF in each patient was determined in chromato -metry method，and serum levels of CA153 were determined in chemoluminescence method. Result：The sensitivity rates of VEGF、CA153 in breast cancer were 60.7%、58.9% respectively，the specific rates were 98.4%、96.3%，and the accuracy rates were 89.8%、88.1% respectively. In combined assay of VEGF，CA153，the sensitivity rate was 92.8%，the specificity rate was 88.8%，and the accuracy rate was 89.8%. Comparing with the single assay，combined analysis had much higher diagnostic sensitivi -ty． Conclusion：The assay of VEGF，CA153 is significant in diagnosis of breast cancer．It can obviously improve the sensitivity rate when VEGF，CA153 are combinedly analyzed． 　　Key words: VEGF； CA153； Breast cancer； Blood plasma 　乳腺癌是一种严重威胁妇女健康的肿瘤，近年来患病率呈明显上升趋势。目前用于临床检测的肿瘤标志物已有许多种，但单一项目的检测因其敏感性、特异性等原因较难达到预期的目的。为观察血管内皮生长因子(Vascular endothelial growth factor，VEGF)、CA153在乳腺癌患者血浆中的表达,提高诊断的敏感性，我们对VEGF、 CA153进行联合检测，探讨它们在乳腺癌诊断中的价值，现将结果报告如下： 　　1 资料和方法 　　1.1 对象与分组： 　　158例乳腺疾病患者均为我院2000年3月至2006年10月住院和门诊的女性病例，其中乳腺癌56例，年龄22～71岁，平均年龄(41.3±11.5)岁；良性乳腺疾病102例，年龄24～73岁，平均年龄(44.6±13.8)岁。均经临床及病理诊断等证实。 　　1.2 试剂及仪器： 　　VEGF试剂盒由深圳晶美生物工程有限公司提供,用上海棱光技术公司生产的722S型分光光度计比色, CA153所用试剂购自美国雅培(ABBOTT)公司，AX-SYM 型化学发光免疫分析系统为美国产品。 　　1.3 检测方法： 　　晨起抽取空腹静脉血，置入含防凝剂的试管，迅速离心提取血浆层