苹果多酚hplc检测条件的优化研究-食品科学.pdfVIP

536 2008, Vol. 29, No. 12 食品科学 ※分析检测 赵超敏，车振明* (西华大学生物工程学院，成都 四川 610039) 本实验高效液相色谱以色谱柱为C18 柱(150mm ×4.6mm ，5 μm) ，流动相为甲醇和醋酸，柱温为40 ℃和 20 ℃，流速为1.00ml/min，线性梯度洗脱，紫外检测器波长为280nm 和320nm 。在此色谱条件下,各组分均得到良 好分离。用乙酸乙酯提取苹果中的酚类物质，在pH 值为2.0 时分离提取酸性酚，在pH 值为7.0 时分离提取中性 酚，通过对中性酚和酸性酚色谱分离条件的摸索和优化，最终选用RP-C 18 色谱柱，中性酚的分离以4% 醋酸缓冲 液和甲醇为流动相，酸性酚的分离以甲醇-4% 醋酸为流动相，建立起酚类物质高效液相色谱测定方法。 苹果；酚类物质；高效液相色谱法 Optimization Study on HPLC Detection Conditions of Apple Polyphenols ZHAO Chao-min ，CHE Zhen-ming* (School of Bioengineering, Xihua University, Chengdu 610039, China) Abstract ：High performance liquid chromatography (HPLC) was used to detect apple polyphenols, and its basic operation conditions were determined as: C18 column (150 ×4.6 mm，5μm), methanol-acetic acid as mobile phase,flow rate 1.00 ml/min, column temperature 40 ℃ and 20 ℃ and wavelength of detector 280 nm and 320 nm. All compositions of apple polyphenols were separated completely under these chromatography conditions. Phenolic compounds were extracted from apples with ethyl acetate. Neutral phenolics were extracted at pH 7.0 and acid phenolics were extracted at pH 2.0. RP-C18 column is suitable to separate apple polyphenols and the mobile phase for the separation of neutral and acid phenolics is identical, the mixture of 4% acetic acid and purified water. Key words ：apple ；phenolics ；high performance liquid chromatography 中图分类号：TS210.7 文献标识码：A 文章编号：1002-6630(2008)12-0536-05 苹果多酚包括多种酚类物质，可分为酚酸及其羟基 1.2 仪器与设备 酸酯类、糖类衍生物和黄酮类化合物(如儿茶素、表儿茶 Agilent 1100 高效液相色谱分析仪、Agilent 1100 化 素、原花青素、二羟基查耳酮、黄酮醇配糖体等) [ 1 - 2 ] 。 学工作站、A g i l e n t 1 1 0 0 V W D 可变波长检测器、 苹果的品种不