螺旋藻多糖含量测定（Spirulina platensis, polysaccharide, content determination）.docVIP

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螺旋藻多糖含量测定（Spirulina platensis, polysaccharide, content determination）.doc

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螺旋藻多糖含量测定（Spirulina platensis, polysaccharide, content determination）

螺旋藻多糖含量测定（Spirulina platensis, polysaccharide, content determination） Spirulina platensis, polysaccharide, content determination Update Date: 2009-12-21 Click: Zhu Ying, Luo Yongming, Yan Xiluan, Cao Lan Abstract: the content of polysaccharide in Spirulina platensis was determined by phenol sulfuric acid method. The method was simple and accurate. The average recovery was 99.71%, and the coefficient of variation was 1.479%. Keywords Spirulina platensis; polysaccharide; content determination; The, Deterimination, of, Polysaccharides, in, Spitulina, Maxima Zhu, Ying, Luo, Yongming, Yan, Xiluan, et, Al Jiangxi, College, of, TCM, Nanchang 330006 Abstract The deterimination of polysaccharides in spitulina maxima was made by a simple and precious method of phenol-sulphuric acid. The average rate of recovery is and variation coefficient is 1.479%. 99.71% Key, words, Spitulina, maxima, Polysaccharides, Deterimination Turpin (Spitulina) belongs to prokaryotic plants of blue-green algae, blue-green algae, algae, and contain very rich nutrients and anti radiation, anti-tumor and regulating immune function of halogen resistant algae. Recent studies have shown that the physiological active substance in Spirulina is the polysaccharide (1). Therefore, the content of polysaccharide was determined by phenol sulfuric acid method. 1 instruments and medicines Type 721 spectrophotometer (Shanghai), 5% (from 100 g phenol phenol solution, and aluminum sheet of 0.1 g and 0.05 g of sodium bicarbonate, distillation, collected 182 C fractions, weighing 10 g, 190 g water, the brown bottle refrigerated), spirulina powder (provided by Nanchang foreign trade company Boyuan varieties Spirulina maxima Spirulina maxima). 2 experimental methods [2] 2.1 extraction of polysaccharides: weigh spirulina powder 100 g, industrial ethanol reflux extraction 2 times, filtering, the residue of enamel disc swing with water bath ethanol, 8 times, 6 times, 4 times the amount of boiling water for 1 h and 40 min res