917例免疫印迹法检测hiv抗体阳性及不确定结果带型分析与讨论.docVIP

917例免疫印迹法检测HIV抗体阳性及不确定结果带型分析与讨论 谢思燕1，陈学杰2 （1.广西医科大学第一附属医院，南宁 530021；2.通讯作者 广西医科大学第一附属医院，南宁 530021） 摘要：目的 回顾性分析2014-2015年广西医科大学第一附属医院艾滋病确证实验室人类免疫缺陷病毒（human immunodeficiency virus ,HIV）抗体初筛阳性标本的确证实验结果，探讨免疫印迹试验（western blot,WB）带型与HIV感染的关系。 方法 对送至本实验室的917例HIV抗体筛查阳性标本，经WB确证后阳性及不确定结果进行整理，用spss-17进行统计分析 。 结果 初筛阳性标本中，确证阳性855 例，阳性率93.2%，确证阴性43 例，阴性率4.7%，不确定结果 19例，占筛查阳性的2.1% 。确证阳性标本中，env带阳性率最高，其中gp160最高为100%，p55阳性率最低，占38.6%；不确定者中，共有8种带型， gp160p24带型出现最多占 31.6%，不确定者中随访到8例，其中6 例结果阳转。HIV-1抗体WB带型分布在不同性别，不同年龄人群间差异无统计学意义 ，pol基因编码的p31和p55蛋白及gag基因编码的p17和p39蛋白在不同年龄组人群间差异有统计学意义。 结论 确证为HIV阳性者各带型出现几率有差别，出现不确定带型，应谨慎处理，加强随访，初筛实验存在一定的假阳性，艾滋病的确诊要结合WB，流行病学和其他实验室检测指标综合判定。 关键词： 艾滋病病毒抗体；免疫印迹试验；带型；不确定结果；随访 Analysis and discussion of 917 cases of positive and indeterminate results of HIV antibody detected by Western blot XIE Si-yan 1 ,CHEN Xue-jie2(1First Affiliated Hospital，Guangxi Medical University, Nanning 530021, China；2 First Affiliated Hospital，Guangxi Medical University, Nanning 530021) Corresponging authority：CHEN Xue-jie，Email：chenxuejie1970@163.com Abstract: Objective Retrospective analysis of the confirmatory test results of human immunodeficiency virus (HIV) antibody screening positive samples from 2014 to 2015 years in the AIDS Prevention Laboratory of the First Affiliated Hospital of Guangxi Medical University ， to explore the relationship between the band pattern of Western blot (WB) and HIV infection. Methods 917 cases of positive and indeterminate results of HIV screening positive samples collected from our laborarory were collated and analyzed by spss-17. Results Among the screened positive samples，855 were confirmed positive，the positive rate was 93.2%，and 43 cases were proved negtive，the negative rate was 4.7%，and the uncertainty was 19，accounting for 2.1%. The positive rate of env band was highest in confirmation of positive samples，among which the highest gp160 was 100%，and p55 positive rate was lowest，accounting for 38.6%. Among the uncertainties，the