甘草化学成分分离细胞培养和分析研究进展-天津中草药杂志社.PDFVIP

• 188 • 现代药物与临床 Drugs Clinic 第 26 卷 第 3 期 2011 年 5 月 甘草化学成分分离、细胞培养和分析研究进展 1, 2 1, 2 3 1, 2 3 3* 陈 超 ，李 宁 ，倪 慧 ，孟大利 ，张 娟 ，贾晓光 1. 沈阳药科大学 中药学院，辽宁 沈阳 110016 2. 沈阳药科大学 基于靶点的药物设计与研究教育部重点实验室，辽宁 沈阳 110016 3. 新疆维吾尔自治区中药民族药研究所，新疆 乌鲁木齐 830002 摘 要：甘草因疗效显著、药理作用明确，一直以来都是研究的热点。近年来有20 个黄酮类化合物和 6 个三萜类化合物被 陆续分离出来，其中包括 16 个新的黄酮类化合物和 3 个新的三萜类化合物。细胞培养是获得甘草有效成分的有效途径之一， 目前应用细胞培养技术主要生产甘草黄酮类成分。综述了近年来从甘草中提取的黄酮类、三萜类化合物，细胞培养方法对甘 草黄酮类成分的影响，以及毛细管电色谱法、HPLC 法、免疫测定法等检测甘草化学成分方法的研究进展。 关键词：甘草；黄酮类；三萜类；细胞培养；分析 中图分类号：R282.71 ；R284.2 文献标志码：A 文章编号：1674 - 5515(2011)03 - 0188 - 07 Advances in studies on chemical constituents of isolation, cell culture, and analysis of Glycyrrhizae Radix et Rhizoma 1, 2 1, 2 3 1, 2 3 3 CHEN Chao , LI Ning , NI Hui , MENG Da-li , ZHANG Juan , JIA Xiao-guang 1. College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016 , China 2. Key Laboratory of Structure-Based Drug Design Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China 3. XinJiang Institute of Chinese Materia Medica and Ethnodrug, Urumqi 830002, China Abstract: Glycyrrhizae Radix et Rhizoma has been the research focus because of its conspicuous healing efficacy and pharmacological activities. Recently, 20 flavonoids and 6 triterpenes compounds, including 16 new flavonoids and 3 new triterpenes have been isolated from this plant. It was also found that cell culture provided an effective way to acquire effective components of Glycyrrhizae Radix et Rhizoma . The cell culture technique is mainly used for product Glycyrrhizae Radix et Rhizoma flavonoids. In this paper, the recent progresses was reported on the chemical components of the plant, the cell culture method for the bioactive f