抑制生长素途径增加对病原菌的敏感程度.pdf

Molecular Plant • Volume 1 • Number 3 • Pages 496–509 • May 2008 RESEARCH ARTICLE Repression of the Auxin Response Pathway Increases Arabidopsis Susceptibility to Necrotrophic Fungi a b ´ a ´ a a Francisco Llorente , Paul Muskett , Andrea Sanchez-Vallet , Gemma Lopez , Brisa Ramos , ´ ´ a ´ a b a,1 Clara Sanchez-Rodrıguez , Lucia Jorda , Jane Parker and Antonio Molina ´ ´ ´ ´ a Centro de Biotecnologıa y Genomica de Plantas (CBGP), Departamento de Biotecnologıa-UPM, ETS Ingenieros Agronomos, Avda Complutense, E-28040 Madrid, Spain ´ b Department of Plant–Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne Weg 10, 50829 Cologne, Germany ABSTRACT In plants, resistance to necrotrophic pathogens depends on the interplay between different hormone sys- tems, such as those regulated by salicylic acid (SA), jasmonic acid (JA), ethylene, and abscisic acid. Repression of auxin signaling by the SA pathway was recently shown to contribute to antibacterial resistance. Here, we demonstrate that Arabidopsis auxin signaling mutants axr1, axr2, and axr6 that have defects in the auxin-stimulated SCF (Skp1–Cullin– F-box) ubiquitination pathway exhibit increased susceptibility to the necrotrophic fungi Plectosphaerella cucumerina and Botrytis cinerea. Also, stabilization of the auxin transcriptional repressor AXR3 that is normally targeted for removal by the SCF-ubiquitin/proteasome machinery occurs