适宜运动训练对反复心理应激大鼠海马脑源性神经-天津体育学院学报.pdfVIP

天津体育学院学报 2009 年第24 卷第1 期 Journal of TUS Vol．24 No．1 2009 75～78 ● 研究报道 Short Comunications 适宜运动训练对反复心理应激大鼠海马脑源性神经营养因子的 影响 尹剑春 摘要：目的：探讨适宜运动训练对心理应激大鼠海马脑源性神经营养因子（BDNF）水平的影响。方法: 对SD 大鼠进行为期8 周，每次60 min 的游 泳运动训练，并在运动后期施加2 周的心理应激，测定大鼠海马CA1 区脑源性神经营养因子（BDNF）积分光密度、平均光密度、阳性物面积，面密 度、阳性细胞数、数密度水平变化。结果: （1）经过2 周心理应激后，心理应激组大鼠海马CA1 区脑源性神经营养因子（BDNF）积分光密度、平均光 密度、阳性物面积，面密度、阳性细胞数、数密度水平显著低于对照组；（2）经过8 周运动训练后，运动组大鼠海马CA1 区脑源性神经营养因子 （BDNF）积分光密度、平均光密度、阳性物面积，面密度、阳性细胞数、数密度水平显著高于对照组；同时运动+ 心理应激组大鼠海马CA1 区脑源 性神经营养因子（BDNF）积分光密度、平均光密度、阳性物面积，面密度、阳性细胞数、数密度水平显著高于应激组；结论：适宜运动训练可以降低 心理应激反应程度,使大脑海马区BDNF 水平提高，维持大脑海马区在应激状态下的生理功能稳定。 关键词：运动；心理应激；海马；脑源性神经营养因子 中图分类号：G 804.7 文献标识码：A 文章编号：1005- 0000 （2009）01- 0075- 04 The Effect of Adapted Exercise on Hippocampus Brain-Derived Neurotrophic Factor in Rats after Mental Stress YIN Jianchun (Dept. of PE, Huaiyin Normal College, Huaian 223001, China) Abstract: Objective: To explore the effect of exercise training on concentration of hippocampus brain-derived neurotrophic factor (BDNF) in mental stress rats. Methods: SD rats were conducted on exercise training with 60 min swimming for 8 weeks and exerted on mental stress for 2 weeks in the later stage of training. Concentration of points of optical density, average optical density, positive substance acreage, acreage density, positive cell, number density of BD－ NF in hippocampus CA1 area were measured. Results: First, after 2 weeks mental stress, points of optical density, average optical density, positive sub－ stance acreage, acreage density, positive cell, number density of BDNF in hippocampus CA1 area was significant lower than that in control group. Second, after 8 weeks training, points of optical density, average optical density, positive substance acreage, acreage density, positive cell, number density of BDNF in hippocampus CA1 area were significant higher than