高效液相色谱法检测红枣中苯甲酸.pdfVIP

质量控制 中国果菜 33 高效液相色谱法检测红枣中苯甲酸 郑晓冬 宋烨 潘少香 孟晓萌 刘雪梅 闫新焕 （中华全国供销合作总社济南果品研究院，山东济南250014） 摘 要 本文建立了一种水溶液直接浸提结合高效液相色谱法检测红枣中苯甲酸的方法。采用剪切 均质 超声相结合的方式提取 院 - - 红枣中的苯甲酸，比较选择了液相色谱流动相类型与配比，检测了红枣样品中苯甲酸的含量，并进行了加标回收实验。结果显示，以 甲醇水（含 乙酸铵） 时，苯甲酸出峰锋型最佳，保留时间合适，分析速度快。该方法对苯甲酸的提取效率高，回收率在 : 0.15% =5:95 78.8%~105%之间，精密度在4.87%~5.42%之间。方法线性良好（2=0.9990），灵敏度高，测定下限为1.5mg/kg，可满足红枣中苯甲酸 的检测需求。 关键词 红枣；苯甲酸；高效液相色谱 院 中图分类号院TS255.3 文献标志码院A 文章编号院1008-1038(2015)10-0033-04 Determination ofBenzoicAcidinJujube by HighPerformance Liquid Chromatography ZHENGXiao-dong SONGYe PANShao-xiang MENGXiao-meng LIUXue-mei YANXin-huan (JinanFruitResearchInstitute,AllChinaFederationofSupplyMarketingCo-operatives,Jinan250014,China) Abstract: In the paper, a water direct extraction combined HPLC method was developed for the determination of benzoic acid injujube. The combination pretreatment of cut-homogenization-ultrasound was adopted. Different influencing factors such as the proportion of mobile phase were studied. Based on this, real samples determination and recovery experiments were carried out. The result showed that the the mobile phases were methanol and 0.15% ammonium acetate aqueous solution(5:95,v/v). Undertheseconditions,thepeak shapewaspresentedwellwith anappropriateretentiontime andrapid 2 measurement.Therecoverieswere 78.8%~105%, andthe RSDswere4.87%~5.42%.The linearity isgood in detection( = 0.9990), andLOQ ( =10)was 1.5mg/kg.Thismethod canmeettheneedforthe quantitativedetectionofbenzoicacidin jujube. Keywords: Jujube; benzoic acid;highperformanceliquid chroma