P-15作为新型生物材料在治疗椎体压缩骨折中的应用的试验研究.PDFVIP

张 郡 呼 春 刘钦毅 摘要 吉林大学第二医院疼痛科，长春，130041 P-1515种氨基酸肽，属于类似人Iα细粘域 。这目前一组织正在生产并使用作为层骨（ABM/P15），在这种形 实验该具有增强细黏，促进细、迁移细生存[1]。打算 在体外培养进一步研究细迁移试验试管定实验。还打算在小鼠模体 内研究讨P15对于管生，软骨内骨骨形影制。结论P15以增强骨 细粘、促进细、迁移高细存活，要以促进骨生。 关键词：P15；骨质；骨；微 责作联系：毅，E-mail: liuqinyi1@ P-15作为新型生物材料在治疗椎体压缩骨折 中的应用的实验研究 Experimental Research on the Application of P-15 in the Treatment of Osteoporosisvertebra Compression Fracture as a New Biological Material Jun Zhang, Chun Hu, Qin-yi Liu Department of Pain, The Second Hospital of Jilin University, Changchun, 130041 Abstract P-15 is the common name of a synthetic 15 amino acid peptide (GTPGPQGIAGQRGVV) which mimics the cell-binding domain of alpha-1 chain of human collagen type I protein. It is currently being used as a tissue engineered product coating the surface of anorganic bone material (ABM/ P15). In this form it has demonstrated the ability to enhance cells adhesion, promote cell differentiation, migration and cell survival. The goal of our previous work was to evaluate if modification of the titanium surface with covalently linked P15 would enhance osteoblastic adhesion, proliferation and maturation, and investigate the mechanism by which this occurs. We propose in vitro investigation into the effects of P15 on using a novel cell migration assay and a tube formation assay. We also propose in vivo study to investigate the use of P15 for the enhancement of angiogenesis, endochondral ossification and bone formation in a subcutaneous mouse model. We found that P15 can enhance osteoblasts adhesion, promote cell differentiation, migratio