甲状腺激素对大鼠睾丸间质细胞再生地影响.pdf

甲状腺激素对大鼠睾丸间质细胞再生地影响.pdf

英文摘要 weretaken frozenat一70℃andslicedwith1 out,quickly 5∥m thicknessLeica microtome.NBTmethodwasused by freezing in of todemonstrate3B-HSD cells.②Function enzymeLeydig takenoutandculturedwith cells:TIhetesteswere Leydig ^ hormone 觑 Vff阳, enzyme luteinizing (LH) magnetic in wasusedtodetectthetestoste!oneleVel immunoassay bloodwasobtainedfrominfe“orVenacaVeand supernate.The usedto Was centrifugated,magneticenzymeimmunoassay detectthetestosteroneleVelinsemm. and ⑨Morphology testesweretakenoue andfixed of cells:111e by quantityLeydig as andstained usual,sliced 10%formalin,imbeddedparaffin by withHE and of cells quantityLeydig staining.Themorphology under wereobserved lightmicroscope. Results: 1ResultsofNBTmethod 1.1 ceUswere positiVe Hyperthyroidismgroup:3B-HSD notobservedat7m small

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