EB病毒BARF1基因对人胃上皮细胞恶性转化的作用.docVIP

EB病毒BARF1基因对人胃上皮细胞恶性转化的作用 李淑英周玲 1河北联合大学基础医学院，河北 唐山063000 2中国疾病预防控制中心病毒病预防控制所肿瘤病毒室，传染病预防控制国家重点实验室病毒性肿瘤组，北京100052 3北京工业大学生命科学与生物工程学院，北京 100124 [摘 要The Role of Epstein-Barr Virus–Encoded BARF1?Gene in?Malignant Transformation of Human?Gastric Epithelial Cells 朗读 显示对应的拉丁字符的拼音 Li Shuying1, Li Jintao2, Du Haijun2, Wang Zhan2, Zhu Lihua1, Zhou Ling2*, Zeng Yi2* 1 College of Basic Medicine, Hebei United University, Tangshan 063000, China; 2 National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (Chinese CDC), and State Key Laboratory for Infectious Disease Prevention and Control (SKLID), Beijing 100052, China. 3 College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, China. [Abstract] Objective: To explore the role of BARF1 gene encoded by Epstein-Barr virus ( EBV ) in?malignant transformation of human?gastric epithelial cells. Methods: BARF1 gene carried by the eukaryotic vector PIRES2-EGFP/BARF1 was transfected into human gastric epithelial cell GES-1, Screened by G418, and obtained human gastric epithelial cells of stable expression BARF1 gene. Colony formation?in soft agar?and?tumorigenicity of?the transfected cells in SCID?mice were observed. Results: The BARF1?gene transfected cells?and that of?TPA?stimulation?formed colonies in soft agar, cloning?rate?was?24.2% (58/240), 40.0% (96/240), respectively,?Without?Transfected cells and?empty vector?transfected?cells?in soft agar did not formed?colony. Tumors were formed in SCID?mice for BARF1?gene in?transfected?cells?and stimulated with?TPA.?Tumors failed to form in SCID?mice for non-transfected?groups,?empty vector?transfected groups and?BARF1?gene?transfected?cell groups?, but nodules in the?SCID?mice were formed for the?BARF1?gene?transfection?cell?groups. Conclusion:?Malignant transformation were induced? by BARF1?gene transfected cells and?obtained the characteristics of?the tumor?cell growth?in BARF1?gene transfected cell