酵母菌吸附重金属铬的生理代谢机理及细胞形貌分析.PDFVIP

第28 卷第 1期 环 境 科 学 Vol. 28,No. 1 2007 年1 月 Jan. ,2007 NVIRONM NTAL SCI NC * 何宝燕, 尹华 , 彭辉, 叶锦韶, 杨峰, 秦华明, 张娜 ( 暨南大学环境工程系, 广州 510632) :从细胞的 理代谢及微观形貌方面研究了1 株酵母菌富集废水中铬的机理. 阳离子运输实验表明: 酵母菌在吸附铬的过 + 2+ + 2+ 程中,伴随着K 、Mg 、Na 、Ca 等阳离子的大量释放, 铬离子和质子的跨膜运输与胞内阳离子释放相耦合; 反应 30 min, 细 胞对铬的吸附接近饱和, 阳离子与铬的交换达到平衡. 同时细胞分泌酰胺和蛋白等有机分子, 作为Cr( Ö) 的还原性物质及离 子主动运输的载体,促进了铬的胞内积累. 原子力显微镜、扫描电镜、X 射线衍射能谱分析吸附铬前后细胞微观形貌、元素含量 变化:细胞表面高毒性铬的沉积、细胞膜通透性的增强、铬与细胞内有机大分子的螯合作用, 及胞内大量维持细胞结构的阳离 子向体外扩散等行为破坏了细胞的微观结构, 致使细胞产 空壳、内陷甚至裂解等现象. :铬; 酵母菌;离子运输; 吸附机理;细胞形貌 :X703 :A :025023301(2007)0120194205 Cell Physiology Metabolism and Morphology Study of Chromium Biosorption by Yeast H Bao2yan,YIN Hua,P NG Hui,Y Jin2shao,YANG Feng,QIN Hua2ming, ZHANGNa ( Department of nvironmental ngineering, Jinan University, Guangzhou 510632, China) Abstract:Physiology metabolism and micromorphology were studied to reveal mechanism of chromium accumulation in wastewater by yeast. + 2+ + 2+ Cation transportation experiment showed that the transportation of Cr, H ion coupled with release of intracellular K ,Mg ,Na ,Ca in the process of biosorption and in 30 min the biosorption approached saturation and the exchange between Cr and cations reached balance. Meanwhile the cell secreted amide and protein which acted as reduction products of chromium ( Ö) and carriers of ion transportation to accelerate the intracellular accumulation of chromium. Atomic force microscopy(AFM), scanning electron microscopy( S M) and energy2 dispersiveX2ray spe