常用缓冲液的配制方法（Method for preparing common buffer）.docVIP

常用缓冲液的配制方法（Method for preparing common buffer） Method for preparing common buffer 1. glycine HCl buffer (0.05M) X, ML, 0.2M, glycine, +Y, ML, 0.2M, hydrochloric acid, and then diluted with water to 200ml PH, X/ml, Y/ml, pH, X/ml, Y/ml 2.250, 44, 3.050, 11.4 2.450, 32.4, 3.250, 8.2 2.650, 24.2, 3.450, 6.4 2.850, 16.8, 3.650, 5 Glycine molecular weight = 75.07 0.2M glycine solution containing 15.01g/L 2. phthalic acid two hydrochloric acid buffer (0.05M) X, ML, 0.2M, phthalate, two formic acid, potassium hydrogen, +Y, ML, 0.2M, hydrochloric acid, and then diluted with water to 200ml PH, X, Y, pH, X, Y 2.25, 4.670, 3.25, 1.470 2.45, 3.960, 3.45, 0.990 2.65, 3.295, 3.65, 0.597 2.85, 2.642, 3.85, 0.263 3.05, 2.032 Potassium hydrogen phthalate, = 2.4.23, 0.2M, two formic acid, potassium hydrogen solution, containing 40.85g/L 3. sodium hydrogen phosphate two sodium citrate buffer PH, 0.2M, Na2HPO4/ml, 0.1M, citric acid, /ml, pH, 0.2M, Na2HPO4/ml, 0.1M, citric acid, /ml 2.2, 0.40, 19.6, 5.2, 10.72, 9.28 2.4, 1.24, 18.76, 5.4, 11.15, 8.85 2.6, 2.18, 17.82, 5.6, 11.60, 8.40 2.8, 3.17, 16.83, 5.8, 12.09, 7.91 3, 4.11, 15.89, 6, 12.63, 7.37 3.2, 4.94, 15.06, 6.2, 13.22, 6.78 3.4, 5.70, 14.30, 6.4, 13.85, 6.15 3.6, 6.44, 13.56, 6.6, 14.55, 5.45 3.8, 7.10, 12.90, 6.8, 15.45, 4.55 4, 7.71, 12.29, 7, 16.47, 3.53 4.2, 8.28, 11.72, 7.2, 17.39, 2.61 4.4, 8.82, 11.18, 7.4, 18.17, 1.83 4.6, 9.35, 10.65, 7.6, 18.73, 1.27 4.8, 9.86, 10.14, 7.8, 19.15, 0.85 5, 10.30, 9.70, 8, 19.45, 0.55 Na2HPO4 molecular weight = 141.98 0.2M solution containing 28.40g/L Na2HPO4 = 2H2O molecular weight = 178.05 0.2M solution containing 35.61g/L C6H8O7 = H2O molecular weight = 210.14 0.1M solution containing 21.01g/L 4. citric acid sodium hydroxide hydrochloric acid buffer PH sodium ion concentration /M, citric acid, C6H8O7, H2O/g, sodium hydroxide, NaOH/g, concentrated hydrochloric acid HCl/ml, final volume /L 2.2, 0.202108416010 3.1, 0.202108311610 3.3, 0.202108310610 4.3, 0.20210834510 5.3, 0.35245144681