过氧化氢的测定方法（Methods for determination of hydrogen peroxide）.docVIP

过氧化氢的测定方法（Methods for determination of hydrogen peroxide） Three, catalase activity - ultraviolet absorption method [principle] H202 has a strong absorption at 240nm wavelengths, and hydrogen peroxide decomposes hydrogen peroxide, reducing the absorbance (A240) of the reaction solution with reaction time. The activity of catalase can be measured according to the changing rate of light absorption. Instruments and appliances [] ultraviolet spectrophotometer; centrifuge; mortar; volumetric flask 250ml1; scale Straw 0.5ml2; 2ML1; 10ml tube 3; constant temperature water bath. [reagent] 0.2mol? L-1pH