生物分离工程八沉析.pptVIP

In this type of precipitation, a metal ion will bind to a part of the protein. An advantage of this type of precipitation is that they have great precipitating power in a dilute solution. These ions can be classified into three groups. Ions such as Mn2+, Fe2+, Co2+, Ni2+, Zn2+, and Cd2+ will bind strongly to carboxylic acids and to nitrogenous compounds. Ca2+, Ba2+, Mg2+, and Pb2+ will mainly bind to carboxylic acids. Finally, Ag+, Hg2+, and Pb 2+ will bind strongly to sulphydryl groups. 金属离子沉析 离子型表面活性剂 十六烷基三甲基季铵盐的溴化物（CTAB） 主要用于沉淀酸性多糖 十二烷基硫酸钠（SDS） 主要用于沉淀膜蛋白和核蛋白 离子型多聚物沉析剂 是一类温和的沉析剂，与目标蛋白成盐。 核