探针药物法评价艾瑞昔布对大鼠CYP2C9酶的影响.pdfVIP

· 190· JournalofPharmaceuticals 中国医药工业杂志Chinese 2015，46(2) 探针药物法评价艾瑞昔布对大鼠CYP2C9酶的影响 贡莹1”，何文娟1，一，王伟美1”，张志清件 (1．河北医科大学第二医院，河北石家庄050000；2．河北医科大学，河北石家庄050000) 摘要：利用探针药物法研究艾瑞昔布对大鼠体内CYP2C9酶的影响。将大鼠随机分为试验组和对照组，每组18只，试验 组经口灌胃艾瑞昔布，对照组经口灌胃等量蒸馏水，连续给药7d(2次／d)。d8，每只大鼠经口灌胃甲苯磺丁脲，并于给 药后24h内眼内眦取血，以HPLC法测定血浆药物浓度，拟合药动学参数，并对两组的药动学参数进行统计学分析。结 磺丁脲的AUC。：。。和AUC。。。显著降低，CL明显增加，表明艾瑞昔布诱导CYP2C9酶的活性，加快甲苯磺丁脲的代谢。 关键词：艾瑞昔布；甲苯磺丁脲；探针药物；CYP2C9；高效液相色谱；药动学 中图分类号：R961；0657．7+2文献标志码：A 文章编号：1001—8255(2015)02-0190．03 EvaluationoftheEffectsofImrecoxibonCYP2C9 inRatsProbe Enzymeby Drug GONG Weimeil”，ZHANG Yin91”，HEWenjuanl’。，WANG Zhiqin91+ Medical (，．TheSecond Medical 050000；2．Hebei 050000) HospitalofHebei University,Shijiazhuang University,Shf，iazhuang ABSTRACT：TheeffectoflmrecoxibonCYP2C9 inratswasevaluated were enzyme byprobedrug．Rats dividedintothetest andthecontrolwith18ratsineach．Ratsintest were randomly group group group given thoseincontrol were volumeofdistilled for7 water,twice d8， intragastrically,andgroup givenequal daily days．On eachratwas tolbutamide blood werecollectedfrom foldswithin24hafter given intragastrically．Thesamples epicanthal administrationof theconcentrationsoftolbutamideweredeterminedHPLC．The tolbutamide，and by ofthetwo werecalculatedandthestatistic was were parameters groups