猪肌肉生长抑制素RNA干涉载体的构建与验证.pdfVIP

2012年22(5) 生物技术 论 著 RESEARCHPAPERS 猪肌肉生长抑制素RNA干涉载体的构建与验证 于永生，罗晓彤，李娜，张树敏4 (吉林省农业科学院畜牧科学分院，吉林公主岭136100) 摘要：目的：构建针对猪肌肉生长抑制素mKNA的RNA干涉载体，并体内验证其有效性。方法：分别体外转染化学合成4个 19 肉中表达外源基因奠定了基础。 关键词：猪；肌肉生长抑制素；小干涉RNA；生肌调节因子；体内验证 中图分类号：Q782 文献标识码：A doi：10．3969／j．issn．1004—31IX．2012．05．103 andVerificationof Vectorof siRNA Construction ExpressionMyostatin YU Shu—min‘ Na，ZHANG Yong—sheng，LUOXiao—tong，LI ofAnimal of (Branch 136100，China) Husbandry，JilinAcademyAgriculturalSciences，Gongzhuling constructofRNAivectorwhichcall interfere and its Abstract：Objective：To specifically myostatintranscriptionprove small to were and transfeetionfetal designed porcine specificity．Method：FourinterferingRNAs(siRNA)targetmyostatin synthesized．Mter fibroblastscell RNA vectom vectors were siRNA，the by by interfering expressionspecificityproveddirectlyinjecting were印nstrueted，and thevectorintothe muscleof siRNAfor ofthe wereachievedandwerelinkedinto quadficeps pig．Result：Twosilencingmyostatingene RNAivector．Thetransient resultsindicatedthattheRNAivector Callsilencethe whichthe porcine expression injection myostatingene。in MSTNmRNAdecreased40％tothecont