Najanalgesin对大鼠神经病理性疼痛脊髓GLT-1的影响.docVIP

论著 眼镜蛇镇痛素najanalgesin对大鼠神经病理性疼痛脊髓GLT-1的影响 林勤剑1，江伟建2，赵树进2 （1.中山大学中山医学院药理学，广东 广州 510080；2.广州军区广州总医院药剂科，广东广州 510010） 摘要：目的 观察L5脊神经结扎（SNL）并剪断术后大鼠鞘内注射眼镜蛇镇痛素najanalgesin对脊髓胶质细胞谷氨酸转运体亚型1（GLT-1）表达的影响，探讨najanalgesin脊髓镇痛机制。方法 采用SNL并剪断方法建立神经病理性疼痛模型，90只健康雄性SD大鼠，体重200±20g，随机分为假手术组(A)、SNL模型组(B)、SNL+ najanalgesin 组(C)、SNL+ 生理盐水对照组(D)4大组，术后第一天开始鞘内分别注射10ul生理盐水(A、D)、40ng·kg-1najanalgesin (C), 每日一次。鞘内注射后1、4、7d取各组大鼠L4~L6脊髓，分别采用RT-PCR和western blot检测GLT1mRNA和蛋白表达改变。结果 与假手术组相比，B、D大鼠GLT1mRNA和蛋白表达先增加再降低, C组大鼠GLT1mRNA和蛋白表达明显增加, 但不随时间变化。与D组相比，C组大鼠第7天GLT1mRNA和蛋白表达明显增高。结论　Najanalgesin可以增加脊髓GLT1mRNA和蛋白表达,可能是其脊髓镇痛机制之一。 关键词：眼镜蛇毒；神经病理性疼痛；胶质细胞谷氨酸转运体1；脊神经结扎 中图分类号：R996.3；R992 文献标识码： 收稿日期： [通讯作者] 赵树进，Email：gzzsjzhs@163.com，Tel：020Influence of najanalgesin from Naja naja atra on GLT-1 in spinal cord of rat in neuropathic pain LIN Qin-jian1, JIAN Wei-jian2, ZHAO Shu-jin2 (1.Department of pharmacology, Zhongshan school of medicine, Sun Yat-sen University, Guangzhou Guangdong 510080,P.R.China; 2.Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou Guangdong 510010, P.R.China) Abstract: 【Objective】 To investigate influence of najanalgesin on glutamate-glia l transporter 1(GLT1) in spinal cord of rats after L5 spinal nerve ligation and transection, and explore the spinal analgesic mechanism of najanalgesin. 【Methods】 Utilized L5 spinal nerve ligation and transection as the neuropathic pain animal model, 90 male SD rats, weighted 200±20g, were randomly divided into 4 groups: sham (A), SNL(B), SNL+najanalgesin(C), SNL+saline(D). Respectively 4 groups were treated with intrathecal injections of 10 ul saline (A and D), 40 ng·kg-1 najanalgesin(C ), qd. The L4~L6 segments of the spinal cord were isolated in 1, 4 and 7 days after intrathecal injections. Expression of GLT-1 was determined by RT-PCR and western blot. 【Results】　Compared to sham group, the expression of GLT1 at group B and D both mRNA and protein level increased firstly and decreased later,