橘黄酮的提取及其抑菌特性研究开发.pdfVIP

化 工 进 展 ·2528 · CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 2016 年第35 卷第8 期 研究开发 橘黄酮的提取及其抑菌特性 赵悦，孙庆元，孙琦 （大连工业大学生物工程学院，辽宁大连 116034 ） 摘要：采用乙醇浸提法从橘子皮中提取黄酮，通过正交试验确定提取的最佳条件为：70% 乙醇为提取剂，固液比 为 1 ︰20(g/mL) ，提取温度是70℃，提取时间2h ，在510nm 处测定橘黄酮质量浓度为0.205g/L ，从橘子皮中提 取的黄酮干重得率为2.938mg/g 。本实验探究橘黄酮的抑菌活性，采用牛津杯法测抑菌作用，结果表明，橘黄酮 对金黄色葡萄球菌、大肠杆菌、黑曲霉、灰葡萄孢菌和黄枝孢菌均有抑制作用，铜和镁离子可分别增强橘黄酮 对细菌和真菌的抑菌作用，低pH 和低温可提高抑菌效果，且优化pH 和温度的橘黄酮剂型降低了最小抑菌浓度 （MIC ）20% ，对金黄色葡萄球菌、大肠杆菌、黑曲霉、灰葡萄孢菌和黄枝孢菌的抑菌作用分别提高了 17%、27% 、 25% 、28% 、19%，高于对氯间二甲苯酚和甲基托布津的抑菌作用，特别是高于对细菌的抑菌作用。 关键词：浸取；橘皮；黄酮；抑菌；优化；生物技术 中图分类号：Q 936 文献标志码：A 文章编号：1000 –6613 （2016 ）08 –2528 –05 DOI ：10.16085/j.issn.1000-6613.2016.08.35 Extraction of flavonoids from Citrus reticulate and their antimicrobial characteristics ZHAO Yue ，SUN Qingyuan ，SUN Qi （School of Biological Engineering，Dalian Polytechnic University ，Dalian 116034 ，Liaoning，China ） Abstract ：The flavone compounds were extracted from citruspeels with a modified ethanol extraction method. The optimum extraction conditions were determined by orthogonal tests as ：70% ethanol as extractant with solid to liquid ratio 1 ︰20(g/mL) at 70 ℃ for 2 hours. The concentration of the flavone compounds were determined with a spectrophotometer at λ=510nm. The results showed that the yield of flavone compounds to the raw materials reached 2.938mg/g，and the ethanol recovery could be up to 94%. The antimicrobial features were also studied. The inhibition of the extracted flavonoids against the growth of Staphylococcus aureus ，E . coli，Botrytis cinere ，and Cladosporiumfulvum was found ，and copper and magnesium ions could enh