共表达生长激素释放激素(GHRH)与垂体腺苷酸环化酶(PACAP)对动物生长的影响.pdfVIP

生物化学与生物物理进展 Progress in Biochemistry and Biop hys ics 2009, 36(6): 761~769 Ectopic Co expression of Growth Hormone Releasing Hormone and Pituitary Adenylate Activating Peptide in Skeletal Muscle Enhance Animal Growth* 1) 1) 3) 1) 1,2)** LIU Song-Cai , DAI Jian-Wei , REN Xiao-Hui , HAO Lin-Lin , ZHANG Yong-Liang (1) 130062, China; Dep artment of Biochemis try and Molec ular Biology, College of Veterinary and A nimal Sc ience, Jilin University, Changchun 2)College of A nimal Sc ience, South China A gric ultural Univ ers ity, Guangzhou 5 10642, China; 3) 30023 1,China) A quatic College, He be i A griculture University, Qinhuangdao Abstract Growth hormone (GHRH) and pitutary adenylate cyclase activating peptide (PACAP) are the members of the PACAP/Glucagon superfamily, who are related in both sequence and function. Their stimulation of GH secretion and animal growth is concerned. A series of expression plasmid, pIRES1-GHRH-PACAP (P-G-P), pIRES1-GHRH (P-G) and pIRES1-PACAP (P-P), were constructed, extracted and purified, then transfected into CHO cell line with Lipofectamine. The expression was examined by RT-PCR, dot-ELISA and Western blotting. The biological activity of expression products was detected in rats. At 8 h after inj ection of transfection supernatant, serum IGF- concentrations in