溶液pH和阳离子对端粒G-四链体DNA形成和结构的影响EffectofpH.PDFVIP

2007 年第65 卷 化 学 学 报 Vol. 65, 2007 第16 期, 1728～1732 ACTA CHIMICA SINICA No. 16, 1728～1732 ·研究简报· 溶液 pH 和阳离子对端粒 G-四链体 DNA 形成和结构的影响 周 江 袁 谷* (北京大学化学与分子工程学院化学生物学系 北京分子科学国家实验室 生物有机与分子工程教育部重点实验室 北京 100871) 摘要 采用电喷雾质谱和圆二色谱研究了溶液pH 和阳离子对人类端粒G- 四链体DNA 的影响. ESI-MS 和CD 谱图表 明, pH 可以引起G- 四链体DNA 的构象转变和离解, 而K＋, NH＋阳离子对G- 四链体DNA 的形成有着重要的促进作用. 4 关键词 端粒; G-四链体DNA; ESI-质谱法; 圆二色谱 Effect of pH and Cations on the Formation and Structure of Human Telomeric G-Quadruplex DNA ZHOU, Jiang YUAN, Gu* (Beijing National Laboratory for Molecular Sciences and Key Laboratory of Bioorganic Chemistry and Molecular Engineer- ing of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871) Abstract Electrospray ionization mass spectrometry and circular dichroism were used to investigate the factors which affect the motif of the human telomeric quadruplex, such as pH and cations. ESI-MS and CD spectra indicated that pH could induce both the conformational transition and the dissociation of the quadru- plex. Moreover, K＋and NH＋ cations play an important role in the formation of the quadruplex. 4 Keywords telomere; G-quadruplex DNA; ESI mass spectrometry; circular dichroism 端粒是染色体末端一类特殊的结构, 它是由富含鸟 近年来, 电喷雾质谱(ESI-MS)被广泛地用来研究各 嘌呤的重复序列构成. 人类端粒 DNA 含有大量 类生物分子的结构和性质, 如蛋白质、双链DNA 、RNA TTA