费氏中华根瘤菌内源质粒与大豆品种_省略_菌株竞争结瘤能力及固氮效率的影响_缪礼鸿.pdf

2002,35(7) : 802- 808 Scientia Agricultura Sinica 缪礼鸿, 周俊初, 郑慧芬 ( , 430070) : 采用发光酶基因( luxAB) 标记技术, 在无菌砂培条件下测定了 氏中华根瘤菌( Sinorhizobiumf redii) 内 源质粒和大豆品种对供试菌株竞争结瘤和固氮效率的影响结果表明, 供试菌株的固氮效率主要由共生质粒所决 定; 而竞争结瘤能力主要取决于其染色体所携带的基因, 内源质粒(包括共生质粒和非共生质粒) 对供试菌株竞争 结瘤能力没有明显的影响菌株的竞争结瘤能力对供试的大豆品种依赖性较小, 品种对不同组合中的菌株竞争结 瘤能力影响效果不同 : 氏中华根瘤菌; 质粒;大豆; 竞争结瘤; 固氮效率 The Influence of Indigenous Plasmids and Soybean Cultivar on the Competitiveness for Nodules of the Strains and Nitrogen-fixing Effectiveness of Sinorhizobium fredii MIAO L-i hong, ZHO Jun-chu, ZHENG Hu-i fen (Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, uhan430070) Abstract: The Influence of indigenous plasmids and soybean cultivar on competitiveness for nodules of the strains and symbiotic effectiveness of Sinorhizobiumf redii strains marked by luxAB genes were tested by pot experiments. The results indicated that nitrogen-fixing efficiency of the tested strains mainly depended on sym- biotic plasmids and the competitiveness of nodulation mainly on the chromosome genes of strain, the indigenous plasmids (including symbiotic and non-symbioticplasmid) and soybean cultivar shown no significant influenceon competitiveness of nodulation of the strains tested. ey words: Sinorhizobiumf redii; Plasmid; Soybean; Competitiveness; Symbiotic effectiveness , (non-pSym) [1] / 0 : Rhizobium leguminosarum bv.phaseoli I II CFN299 [ 2] [3,4] [7] , [ 5, 6] pH Mesorhizobium loti ,