藤黄酸_重组高密度脂蛋白纳米粒的制备及性质研究_刘聪燕.pdf

* * ， 210009 GA-rHDL-NPs GA-rHDL-NPs Zeta GA-rHDL-NPs GA-rHDL-NPs 113.53 2.50nmZeta -29.48 0.05mV95.30 0.37%8.51 0.95% GA-rHDL-NPs 4 GA-rHDL-NPs GA-rHDL-NPs A- Study on Preparation and Properties of Gambogic Acid-Loaded Reconstituted High Density Lipoprotein Nanoparticles No. No. 20110096120003No. JKP2011007 * Tel: 025ax: 025-mail: zhoujianp60@163.com Tel: 025ax: 025-mail: wangcpu@ * * LIU Cong-yan, WANG Wei , DING Yang, ZHOU Xin, ZHOU Jian-ping (Department of Pharmaceutics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China) ABSTRACT OBJECTIVE To prepare and study the properties of gambogic acid-loaded reconstituted high density lipoprotein nanoparticles (GA-rHDL-NPs) in vitro and in vivo. METHODS GA-rHDL-NPs were prepared by sodium cholate method. Transmission electron microscope (TEM) was used to observe the morphology of GA-rHDL-NPs, the particle size and Zeta potential of them were measured by laser particle size analyzer, the entrapment efficiency (EE) and drug loading efficiency (DLE) were determined by centrifugal ultrafiltration-HPLC method. The appearance and stability in vitro of GA-rHDL-NPs were investigated. The hemolysis and rabbit ear vein irritation tests were performed to evaluate the safety profiles on intravenous injection of GA-rHDL-NPs. RESULTS GA-rHDL-NPs displayed regular spherical-like shape with particle size (113.53 ± 2.50) nm, Zeta potential (-29.48 ± 0.05) mV, EE (95.30 ± 0.37) % and