结肠癌转移相关基因1 对结肠癌细胞肝转移的影响.pdfVIP

肿瘤2011 年6 月第31 卷第6 期　Tumor Vol. 31, J une 2011　　 513 DOI:10.3781/j .issn.1000-7431.2011.06.007 Basic Research ·基础研究 结肠癌转移相关基 因1对结肠癌细胞肝转移的影响 1 2 张　言 ，王　争 1. 上海市黄浦区中心医院普外科，上海200002 ；2 . 上海市交通大学医学院附属仁济医院普外科，上海200127 [ 摘要 ]　目的：研究结肠癌转移相关基因 1 （metastasis-associated in colon cancer 1, MA CC1 ）的表达对结肠癌肝转 移的影响。方法：将融合绿色荧光蛋白 （green fluorescent proteins ，GFP ）的慢病毒表达载体分别感染具有不同转 移潜能的人结肠癌细胞株 SW 1116 和 HCT 116 。给予 BALB/c-nu/nu 裸 鼠脾脏注射感染后的结肠癌细胞，构建结 肠癌肝转移模型。注射后 35 d ，在荧光解剖显微镜下观察肝脏内结肠癌细胞的转移情况，计算肝转移率。应用蛋 白质印迹法检测 SW 1116 和 HCT 116 细胞中MACC1 蛋 白的表达水平，评估 MACC1 的表达水平与肝转移率的 关系。结果：SW 1116 细胞的肝转移率明显高于 HCT 116 细胞。MACC1 蛋白在 HCT 116 细胞中呈低表达，而在 SW 1116 细胞中呈高表达，差异有统计学意义 （P ＜ 0.01 ）。结论：MACC1 蛋白的表达水平可能与结肠癌的肝转 移能力呈正相关。 [ 关键词 ]　结肠肿瘤；肿瘤转移；动物实验；结肠癌转移相关基因1；细胞，SW 1116 ；细胞，HCT 116 1 [中图分类号]　R735.2 [文献标志码] A [文章编号]　 000-7431(2011)06-513-04 The influence of MACC1 on liver metastasis from colon cancer cells 1 2 ZHANG Yan , WANG Zheng 1. Department of General Surgery, Central Hospital of Huangpu District, Shanghai 200002, China; 2. Department of General Surgery, Renji Hospital, Shanghai Jaotong University School of Medicine, Shanghai 200127, China [A BSTRACT]　Objective: To investigate the effect of expression of metastasis-associated in colon cancer 1 (MACC 1) on the liver metastasis from colon cancer. Methods: The SW1116 and HCT116 cells, which had different liver metastasis abilities, were infected with lentiviral vector pCDH cDNA/ green fluorescent protein (GFP) used as a tracer. The liver metastasis model in BALB/c-nu/nu nude mice was established by spleen injection of these two infected colon cancer cell lines. The liver metastasis from colon cancer cells was observed under a fluorescence dissecting microscope, and the liver metastasis r