绿僵菌钙传感蛋白基因克隆及其与致病性的关系.pdfVIP

2012-04-25 DOI: 10.3724/SP.J.1145.2012.00240 / Chin J Appl Environ Biol 2012 18 ( 2 ) : 240~244 应用与环境生物学报 ， 绿僵菌钙传感蛋白基因克隆及其 与致病性的关系* ** 李 敏 曹月青 （重庆大学生物工程学院基因工程中心，重庆市杀虫真菌生物农药工程中心 重庆 400044） 摘 要 通过筛选文库获得绿僵菌钙传感蛋白基因MaNcs1基因全长cDNA序列，并对其在致病过程中的作用进行了分 析. 结果显示，MaNcs1基因cDNA全长792 bp，开放阅读框为573 bp，编码190个氨基酸；采用RNA干扰技术下调该基因 的表达后，对东亚飞蝗的生测实验表明，绿僵菌毒力显著下降，说明该基因与绿僵菌的致病性有关，为以后深入研究 该基因的致病机制奠定了基础 . 图7 参 14 关键词 绿僵菌；MaNcs1 ；钙传感蛋白；RNA干扰；致病性；毒力 CLS S476.12 : Q785 Cloning of MaNcs1 Gene and Its Role in Pathogenicity of Metarhizium acridum* LI M�� CAO Y��q��g** (Genetic Engineering Research Center, Institute of Bioengineering, Chongqing University; Chongqing Engineering Research Center for Fungal Insecticides, Ch��gq��g 400044, Ch���) Abstract MaNcs-1 g��� w�� c�����d by �c������g th� cDNA ���b���y �f Metarhizium acridum , ��d �t� ����� �� th� p�th�g���c�ty �f M. acridum w�� ����� ��v��t�g�t�d. Th� ������t� �h�w�d th�t th� MaNcs1 cDNA h�d 792 bp �� f����� ����gth c��t�����g �� �p�� ���d��g f����� (ORF) �f 573 bp, wh�ch ��c�d�d � p��t��� w�th 190 ������ �c�d ����d���. C���p���d w�th th� w���d typ� �t����, th� v�������c� �f M. acridum �g����t Locusta migratoria manilensis (Meyen) was significantly decreased when the MaNcs1 g��� w�� d�w�-��g����t�d ����g RNA ��t��f����c�, ��d�c�t��g th�t th� MaNcs1 g��� w�� ��v���v�d �� th� p�th�g������ �f M. acridum . Th��� ������t� w����d c��t��b�t� t� th� f��th�� �t�dy �� d�pth �� �t� p�th�g���c ���ch������ �� M. acridum . F�g 7, R�f 14 K�yw��d � Metarhizium acridum ; MaNcs1; c���c���� ������ NCS-1 g���; RNA ��t��f����c�; p�th�g���c�ty; v�������c� CLS S476.12 : Q785 昆虫病原真菌绿僵