气相色谱-质谱联用返抹测定广玉兰果壳和种子中挥发性化学成分(已发表).doc

气相色谱-质谱联用法测定广玉兰果壳和种子中挥发性化学成分 范小春1，2，梁光义1，2*，曹佩雪2，王道平2 1 贵阳中医学院，贵阳 550002； 2贵州省、中国科学院天然产物化学重点实验室， 贵阳 550002 摘要：目的: 分析广玉兰果壳和种子的挥发性成分，并比较其异同与含量差异。方法：采用水蒸气蒸馏法分别提取果壳和种子中的挥发性成分，用气相色谱-质谱对其挥发性成分进行鉴定。结果：从广玉兰果壳中分离出111种组分，共鉴定了79种化合物，占挥发油总量的56.54%；从种子中分离出88种组分，共鉴定了58种化合物，占挥发油总量的46.02%；其中果壳和种子的共有成分21种（果壳占总挥发油含量的28.46%，种子占33.77%）。结论：为进一步研究开发广玉兰的果壳与种子提供依据。 关键词：广玉兰；挥发性成分；气质联用（GC-MS） Analysis of Volatile Chemical Constituents in Hull and Seeds of Magnolia grandiflora by GC-MS Spectrometry FAN Xiao-chun1, 2, LIANG Guang-yi1, 2*, CAO Pei-xue,WANG Dao-ping Guiyang College of Traditional Chinese Medicine，Guiyang 550002， China 2. The key laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guizhou Guiyang 550002） ABSTRACT：OBJECTIVE: To analyse and compare the volatile components which were extracted from hull and seed of Magnolia grandiflora . METHODS: The volatile components were extracted from hull and seeds of Magnolia grandiflora by steam distillation and the chemical components of them were identified by GC-MS .RESULTS : One hundred and eleven compounds were extracted from the hull, seventy-eight compounds were identified, accounting for 56.54%of the total volatile oil, and eighty-nine compounds were extracted from seed , fifty-eight compounds were identified, accounting for 46.02 % of the total volatile oil. Twenty-one compounds were common in both of hull and seeds. CONCLUSION:The results of the studies can be referenced when the hull and seeds of Magnolia grandiflora need to be further studied. Key words：Magnolia grandiflora；Volatile Chemical Constituents；GC-MS 广玉兰（Magnolia grandiflora）为木兰科木兰属植物，又称大花玉兰、洋玉兰、玉兰，主产地为我国长江流域以南地带。广玉兰叶、花、种子、果壳均可入药，其叶及花中含有挥发油、木兰花碱等多种化学成分，果实中含有脂肪酸，花、叶可提取香料，种子可榨油，其叶乙酸乙酯提取物中还含有黄酮类和酚类化合物，叶和果实中含有五种倍半萜内酯[1-2]。其幼果水提取物含生物碱、皂苷及黄酮苷，乙醇提取物含黄酮和强心苷[3-4]。文献研究未发现有对广玉兰果壳和种子中挥发性成分进行对比性研究。本文采用水蒸气蒸馏法提取广玉兰果壳和种子中的挥发性成分，并用气相色谱-质谱联用鉴定其成分，进行对比分析果壳和种子中的所含挥发性成分差异，为进一步研究开发广玉兰的果壳与种子提供依据。 1 实验部分 1．1 仪器与材料 广玉兰果实购于贵阳市万东桥药材市场（产地：贵州）