信号转导研与究方法.pptVIP

* * ProteinG-Sepharose * RNA提取 甲醛变性电泳 印迹转移 预杂交 杂交（变性探针） 洗膜 放射自显影或化学发光 RNase protection assay (RPA) Sensitive and quantitative alternative to Northern blots for the measurement of gene expression levels. Labelled antisense cRNA is transcribed from a DNA clone in an appropriate vector, hybridized with an mRNA sample, and single stranded RNA digested away with RNase, then run out on a gel. The amount of labelled RNA surviving is directly proportional to the amount of target mRNA present in the sample. 萤火虫荧光素酶(firefly luciferase) (Luc) 荧光素酶可以催化荧光素luciferin氧化成